mminhas44 Posted April 20, 2011 Share Posted April 20, 2011 We have a repeated problem, I haven't seen it to occur so frequently at other places. Our slides, especially marrow and touch imprint slides, get understained. we have tried changing staining time, re-making stock solution but the problem is still there.Does anyone have any logarithm for it ? Link to comment Share on other sites More sharing options...
khalidm3 Posted August 6, 2011 Share Posted August 6, 2011 25 years back, I was in a general lab and faced this problem. I remember if the stain was prepared according to recommended concentration 150gram powder in 1000 Methanol; we we facing similar problem. I remember we we preparing a saturated solution of Leishman stain and were getting ideal results for smears. Link to comment Share on other sites More sharing options...
FAIZAL MAHROOF Posted November 25, 2011 Share Posted November 25, 2011 U can mix Gently the stain before use or get it filtered from a filter paper. Link to comment Share on other sites More sharing options...
Auntie-D Posted November 25, 2011 Share Posted November 25, 2011 Are you making the Leishmans up from powder? If so, how long are you leaving it to 'ripen'? It needs a minimum of 6 weeks in a dark cupboard for optimal staining. As Khalidm3 stated - it also needs to be a saturated solution - this will mean it will need filtering before use.Another thing to look at is how the slides are prepared - slow drying time can make stain uptake poor, as can heavily bloodstained preps. Link to comment Share on other sites More sharing options...
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