QC

[lef5501]

Two quick questions about QC,

The first is about gel QC: Does anyone do a QC on the diluent used to make up cell suspensions? We are using one of these diluents to make up suspensions for DAT testing and I was just wondering if we needed to QC that diluent.

The second is about tube QC: How are people QC'ing the C3 reagent? We QC our poly and IgG on our daily QC, but as of yet we do not QC our C3. Any ideas on both of these would be greatly appreciated.

Thanks.

[SusanM]

We QC the Diluent 2 daily, making a 0.8% suspension of DAT pos and DAT neg cells.

We only QC poly and anti-comp when used, and for the anti-comp we have complement control cells.

Susan

[Lcsmrz]

We run diluent against screen cells to show that the bottle is not contaminated with something that would cause false positives. The same wells show that the gel is not causing false positives with the screen cells.

In tube testing, we run C3-coated check cells after performing a DAT with Anti-C3, but only on days that we perform testing with Anti-C3 reagent. We don't have poly-AHG any more.

[Mabel Adams]

We run a gel xm QC to test the diluent, testing a negative patient plasma sample and our reagent QC kit positive serum sample against a unit made up to 0.8% in the Diluent 2.

We don't do gel DATs. They seemed to be almost too sensitive for general antibody ID workups. When working up transfusion reactions, that sensitivity might be more appreciated.