Posted January 18, 20232 yr comment_84824 Hi, Does anyone have a way to freeze reagent red cells that does not involve Glycerolyte 57, liquid nitrogen or glycegel. we currently use Glycerolyte57 but can only order it by the case and usually only use one bottle a year. Thanks,
January 19, 20232 yr comment_84831 15 hours ago, alt5575 said: Hi, Does anyone have a way to freeze reagent red cells that does not involve Glycerolyte 57, liquid nitrogen or glycegel. we currently use Glycerolyte57 but can only order it by the case and usually only use one bottle a year. Thanks, What type of freezer are you planning to use ?
January 20, 20232 yr comment_84842 this is what's in our freezing "solution" - but we freeze them in liquid nitrogen (they look like "Dip-n-Dots" ice cream Dissolve 15.4 g sucrose, 5.4g dextrose (D-glucose) and 0.29g of sodium chloride in 100ml of distilled water. Mix well in a 250 ml flask.
February 22, 20232 yr Author comment_85026 Thanks, but we are trying to avoid using liquid nitrogen. We are a small blood bank and do not have the space for this type of system and also not enough rare cells to accommodate the cost.
February 24, 20232 yr comment_85038 I think you can just use basic 40% glycerol (in water). Glycerol is available from many sources. Use an equal volume of 40% glycerol to washed, packed red cells. Freeze @ -80 C is small aliquots. I don't remember the details of recovery, but it involves washing with a couple of different Saline concentrations, akin to deglycerolizing red cells for transfusion. The "Valeri Method" - see the article below. Vox Sang 2000;79(3):168-74. An experiment with glycerol-frozen red blood cells stored at -80 degrees C for up to 37 years. C R Valeri 1, G Ragno, L E Pivacek, G P Cassidy, R Srey, M Hansson-Wicher, M E Leavy
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