Jump to content

Freezing of reagent red cells


Recommended Posts

Hi,

Does anyone have a way to freeze reagent red cells that does not involve Glycerolyte 57, liquid nitrogen or glycegel.  we currently use Glycerolyte57 but can only order it by the case and usually only use one bottle a year.

 

Thanks,

Link to comment
Share on other sites


15 hours ago, alt5575 said:

Hi,

Does anyone have a way to freeze reagent red cells that does not involve Glycerolyte 57, liquid nitrogen or glycegel.  we currently use Glycerolyte57 but can only order it by the case and usually only use one bottle a year.

 

Thanks,

What type of freezer are you planning to use ?

Link to comment
Share on other sites

this is what's in our freezing "solution" -  but we freeze them in liquid nitrogen (they look like "Dip-n-Dots" ice cream :D

Dissolve 15.4 g sucrose, 5.4g dextrose (D-glucose) and 0.29g of sodium chloride in 100ml of distilled water. Mix well in a 250 ml flask.

Link to comment
Share on other sites

  • 1 month later...
I think you can just use basic 40% glycerol (in water). Glycerol is available from many sources. Use an equal volume of 40% glycerol to washed, packed red cells. Freeze @ -80 C is small aliquots. I don't remember the details of recovery, but it involves washing with a couple of different Saline concentrations, akin to deglycerolizing red cells for transfusion. The "Valeri Method" - see the article below.
 
Vox Sang 2000;79(3):168-74.  An experiment with glycerol-frozen red blood cells stored at -80 degrees C for up to 37 years. C R Valeri 1G RagnoL E PivacekG P CassidyR SreyM Hansson-WicherM E Leavy
 
Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
  • Advertisement

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.