exlimey
Content Type
Store
Profiles
Forums
Blogs
Events
Frequently Asked Questions
Gallery
Downloads
Glossary
Links Directory
Questions
Jobs
Vendors
Everything posted by exlimey
-
If you are using a commercial anti-D reagent as your starting material, you might be creating your own problems. Most reagents are now monoclonal and as such they often have very peculiar formulations (additives, diluents, etc.) that ensure their stability. When end users dilute or otherwise modify the reagents, they may lose the key element required for reactivity and/or stability (even when frozen). I suggest you try a polyclonal (human) source (which is probably what Malcolm's group is using). If you need to dilute it to get to the desired reactivity, I recommend using either inert normal human serum or 6% BSA. Both diluents should maximize your chances of stability, either in the liquid state or frozen.
-
Multiple Myeloma Therapeutic agent Darzalex interfering with testing
exlimey replied to ElinF's topic in Transfusion Services
Winter - in the case of DARA patients, the DTT is use to treat (panel or screening) cells rather than the serum. SMILLER - do you enzyme-treat your own cells ? If so, have you determined their stability ? -
You will probably need to "qualify" the vendor is some fashion, but I don't believe you have to do any serological comparisons providing you are going to use the material according to the manufacturer's directions.
