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Diane

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Everything posted by Diane

  1. I'm not sure I understand what you are asking, but we use Ortho's "Fetalscreen" kit to detect the presence of fetal cells in Mom. If you were thinking about using weak D testing to detect fetal bleed, the weak D test is no longer considered acceptable for that purpose. Hope this helps.
  2. The 3 day rule is also required by the FDA, for antiglobuling testing. We do the antibody screen within 72. Crossmatches we go as far as 14 days, unless we have to do AHG crossmatches. I believe what they are addressing here is the reactivity of the IgG antibodies in the patient's sample, and that it may decrease after 72 hrs. An immediate spin crossmatch is really an ABO match, so that's considered OK beyond 72 hrs.
  3. With the huge price increases in typing sera, we are considering dropping some of the ones that are usually considered clinically insignificant. Specifically, these would be P1, M, N, Le(a), & I'm considering e also, since we don't have a large enough inventory to find e= units, & always order them from Red Cross anyway. I'm wondering if anyone else has done the same thing. We rarely see these antibodies anymore anyway, since we've been using Gel testing. I'd appreciate any input.
  4. We enter ABO & Rh reactions directly & immediately into the computer so that we can take advantage of the computer system's "Calc Hook" as a check. All other reactions, including a recheck of the ABO/Rh, are written on a worksheet & then final interpretations are copied into the computer.
  5. I am trying to advise a very small hospital in our area that wants to make a change in their compatibilty testing technique (switching from serum to plasma). They probably do 0-2 crossmatches a day. Does anyone know a required # of specimens that they should run to validate the switch?
  6. We were taught when trained on the Ortho gel, that it is OK if the cell suspension goes into the neck of the gel tube, but to remember that if there is a reaction in that tube, you might see a mixed cell picture, since some of the red cells would not have incubated with the pt plasma & would therefore go to the bottom of the gel. Our trainor did NOT address when I manage occasionally to shoot the plasma past the cells & into the neck of the tube. I have decided that, since there is such a tiny volume of plasma, this is NOT OK. It would mean that most of the cells did not incubate with the plasma.
  7. I will just add that we use Ortho reagents, and the increase quoted by our rep was 150% for tube testing reagents. He then suggested that we purchase their new instrument, which would then give us free reagents. I suspect they are trying to force us into Gel and into their instruments. :mad: We are already using Gel, so I would think we should get a price break on the tube testing reagents, but not so. I sure can't drop tube testing altogether. Special techniques are still done by tube, and I also don't want to wait 10 minutes to get a blood type on a trauma patient!!! Diane Shepherd Bay Area Hospital Coos Bay, OR
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