To all Blood Bankers: This question is related to the use of diluent to make 2% suspension of RBC for Antibody Titeration. Is any one using 22% Albumin to make 2% RBC suspension for Antibody Titration? If so, How is the suspension made? The 16th edition AABB Manual does not specify to use albumin. In the14th edition AABB Manual, albumin was allowed to make a suspension. When technical manual does not specify the albumin, I presume only saline can be used. (My old policy,written by former supervisor, has suspension made by albumin) I validated the SOP by using Albumin and normal saline to make the 2 % suspension and albumin gave one titer lower than the saline. Reference the SOP 16th Edition AABB Technical Manual page # 938-939. Thank you in advance for all the input. Urvashi MLS (ASCP) Blood Bank Manager at JCMC