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About Immunohematologist

  • Birthday 02/07/1983

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  1. What do you use as a "lot to lot"? Do you only QC them? and do you document that you QC'ed it compared to the previous lot? My job has a chart that they make us fill out when we change lots. It compares old lot QC passing to new lot QC passing. I think that this is not useful because it isn't really proving anything, since you are only running QC, you are not really comparing the lots.
  2. I believe you will need to correlate both methods with another source. I am not sure where you live, but in my state they are very strict with basically everything. If you never did testing at all before, you may have to do testing and send some samples to another lab to prove they are correct. Once one method is tested and validated, you can use that to test the other method.
  3. The facility that I currently work at has gone crazy with their rules and regulations. I was wondering if there are any other labs out there that do parallel testing with a patient on blood banking regents. I remember when working in another facility, all we had to do was QC new lots or newly opened bottles. I know that parallel testing is a common procedure in chemistry amongst many other departments, but I never really seen a specific "lot to lot" procedure in a blood bank.
  4. I had something similiar to this happen to me. I work at a reference lab, which does not get any patient information besides name, age and sex. So, it is pretty difficult to determine what problem you could be facing with a patient. Our machine basically runs the show, you put the samples on, it does type and screen, and the panels. At the end of the day, i see a sample that is Rh positive with an Anti-D antibody. Ever since this, I have been researching to try to figure out how this could be since there was no error in handling. We use the capture plates, not gels, but I did run a titer on a gel and it was 1:2. All reactions for the panel were 4+ and clearly could not be any other antibody except D. I repeated the type on tubes, and it had a 2+ reaction for D4, screening cells were 2+ and 3+ so, all the reactions were quite strong. We don't have the ability to do elutions or anything fancy like that, but I was just curious of what this could be. I too, thought it may be an auto Anti-D but I don't know for sure.
  5. I am just wondering how it could be possible for a patient to have an Anti-D antibody and be Rh positive. To me, it doesn't make sense since the red cells have the correct antigens, with exposure, it should not induce a production of Anti-D antibodies. I have been trying to figure this out all day. I suppose if the person was weak D positive, then a response could be made from those that were not positive. I'm so confused
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