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Everything posted by profbaud
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We do a Forward blood type on all infants from group O moms and Rh neg moms. If the Mom has a negative Antibody screen and if the baby is an A or B born to an O mom, then we just do an IAT on A or B reverse cells. That determines if Mom's Anti-A, Anti-B or Anti-A,B has crossed the placenta. A Lui freeze is outdated.
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We leave our screening cells out for at least 8-12 hrs a day. we cut the box ]that the reagents come in] in half and use that as a cover over the reagents in the reagent rack. We also keep the provue door closed during the day since it is tinted.
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The SBB exam covers more than the material in the Technical manual and the standards. It includes some Coagulation [disorders, how to distinguish platelet disorders from factor disorders], FDA regulations, Management math problems, regulations for deferring a donor, HLA testing, and lots more. Many moons ago, techs with alot of experience in blood banking could take the exam and pass but the pass rate for them now is less than 50%! If you want to be an SBB, the best thing to do is enroll in an online SBB program like the one at Rush University [i went thru their program and i know teach parttime] and even though I have 30 years experience in Blood Banking, I know I couldn't have passed the exam with out taking this program. Good luck. If you don't pass email me about Rush's program. There are 4 other online programs in US too. The pass rate for taking a program is 95%.
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We perform QC on Polyspecific AHG when we get a DAT order on an adult and its good for 24 hrs from the time it is done. We use 3 cells: 3% Selectogen I [Negative control], Coombs control cells [Positive control for IgG component] and C3 check cells [Positive control for the C3d component]. We spin the tubes at immediate spin and after 5 minute incubation. The Coombs control cells are 3-4+ at IS, the C3d cells can be 0-1+ at IS and 1-2+ at 5 minutes, depending on the age of the check cells and the SI cells remain negative at IS and 5 minutes and then positive when Coombs check cells added. Hope this helps.
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We had this problem too. When I investigated, i found that Ortho uses a mixture of clones for the ABO Gel cards versus their Anti-D typing reagent. We would get a positive on provue and neg when we did the retype using tube method. We then switched to Immucor Anti-D for Rh typing on bench and it matched the provue results. Ortho KNOWS that there is a problem with their typing sera but won't admit it. When we have a discrepancy between Rh types, we have done a weak D test on new negatives that were previous positives and they always reacted at AHG. Either the patient has less D antigens on cells or change in strength of testing reagents. Tell the doctors that the ONLY pateint that will develop Anti-D is the partial D patient, which is rare. Weak D patients won't develop Anti-D
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Cryo 10 packs are pooled together using a sterile docking procedure so the expiration date should be the same as thawing 1 unit of Cryo, which is 6 hrs. I don't know of anything in writing that supports this thinking.
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If you have not taken a blood bank course in school, I recommend you purchase Denise Harmening's Immunhematology text book called Modern blood bank and transfusion practices from F.A.Davis publishers, in addition to reviewing the AABB technical manual. The technical manual alone is not a good resource if you have never had a blood bank course before since it doesn't go into basic knowledge.