yolis76
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Everything posted by yolis76
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Hello all - I wonder if anyone has experience with bringing MLS/MT staff to your labs using the H1b Visa sponsorship route. The paralegal at my organization is asking me to justify the needs for a bachelor's degree for each of the job components listed in our job description for the MT/MLS role. Did you need to do this as well and if so, we can talk to see how you were able to justify? Thanks!
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Thanks for that feedback it's very useful! I am going to buy one to try them out and see how far we can extend the validation. So did you validate it both ways, open and close or just open? I can see AABB coming in and saying, well if the majority of the time it's closed, how can you verify it's not getting TOO cold? Ugh! AABB can be so frustrating!
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Thank you for that feedback! I emailed out this same question to colleagues in the area and they pretty much said the same, and that their maintenence department seems to like them. I'm going to order 2 to replace a double wide jewett like yours. I hope it works out well! :-)
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Is this the one you're using? Click here I want to purchase to try it out but I want to be sure it's the same one you're referring too. What are you using to keep cold inside, bags of ice? Cooler packs? And how many? Thank you!
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It's still only 60% and that's if you go through an accredited program. If you do not then the stat drops to 35%.
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Solid Phase reactions in Newborn with no Maternal history
yolis76 replied to acrolabgeek's topic in Transfusion Services
Having worked with Solid phase long enough I would first suspect junk reactivity and test in the tube with LISS. If that is negative I'd call this a negative screen. If you get something there then it's possible that what someone above suggested - the titer in the mother is just too low to detect and you are picking something up in the infant. -
NO idea.... but I am glad to hear that they are wrong!
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Thanks! I was concerned. I am working with Dr. Harmening and she was told by someone on the west coast that 'forward' and 'reverse' typing were old terms... but I am currently an educator in both an SBB program and an MLS program and I use them all the time :-)
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If a DCe/DcE patient receives Rh negative blood (usually dce/dce)then they may develop anti-f ? --> I would say yes because the recipient does not have the 'f' antigen (you need to remember that the ce antigens must be inherited from the same haplotype ie the same parent); the donor does express the 'f'antigen, therefore the recipient may form Anti-f. The Rh genotypes DCe/DCe and DCe/DcE can make anti-f whereas DCe/dce, DcE/dce and dce/dce can cause the anti-f to be made if transfused into the first two genotypes? --> yes, see above. What about auto anti-f. Is there a tendency of any paticular genotype to make it more often? It occurs often with anti-c? --> not sure but there's just about auto-anti-anytying... Marion Reed's book might give you a better idea about the frequency of this occurring.
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Is it true that the terms "forward" and "reverse" typing are no longer used, acceptable terms for ABO typing?
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I've seen Anti-E and Anti-C in WinRho and IVIG. But I don't think I've seen it from RhIg...
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Well seeing how some people have confessed to embarrassing mistakes on their part, I will confess mine. In my defense, I was not even an MT student yet, but I had started to work in the Micro lab doing receiving samples and doing simple setups. I started working there over the summer before the MT course began so I was very very fresh.... A doctor and her resident walk in and hand me a sample and say: I'd like EB testing done on this please" And I look at her and say: Ebola virus? No she says, Epstein Barr Virus... how stupid did I feel?!
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I've had this exact same thing happen to me. I was amazed!
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ABO discrepancy; unusual results. What do you think...?
yolis76 replied to Fluffy agglutinates's topic in Case Studies
Any results for this case? -
How many times do you perform an antibody identification?
yolis76 replied to javvcr's topic in Transfusion Services
We do identification every 14 days. It's too much work to do them every 3 days. We compare current screen results with the previous result, and perform the DAT. If there has been no significant change in the screen (a prev neg cell is now positive) and if the DAT continues to be neg, we do not run identification until after 14 days. If the previous DAT was positive, we only work it up IF the current DAT is stronger than the previous one, and that is only done IF the patient was transfused between the last DAT and the current DAT. Hope this makes sense. YS -
yes but nothing for purchase.....
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How do you quantitate the anitbody? I agree, blocking phenomenon....
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Back in 2002 PBS showed a 4 part documentary called: Red Gold, The Epic Story of Blood... I know its available for sale on thirteen.com but I was just curious if any one out there has a copy I can borrow! It's expensive on the web site, and they've never aired it again on PBS. It was a great documentary for us Blood Bank nerds, and I would love to show it to my students (although I think they would hate me!) Thanks!
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The reactivity seen at AHG could be carryover from the strong reactions at IS... I would have to see the reaction strengths to see if this is possible. But at the same time, we use Solid phase, and because this methodology claims to only pick up IgG antibodies, we identify a few Anti-Ms a year using SP. If we test these same patients using tube you can clearly see its only a cold reacting anitbody, so why are we seeing reactivity in SP? The manufacturer has suggested that the indicator cells express M antigen, and therefore the M antigen on the indicator cells is causing the reactivity. I have done some additional testing to see if this is the case, but I have not had conclusive results. We don't have any DTT to test the patient serum and see if we can separate IgG from IgM. Our medical director is wary about not giving M neg units, so if the Anti-M reacts in SP we automatically give M neg units with AHG XM, even if the tube testing clearly shows cold antibody.
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I also think that if you are going to perform Gel titers you need to make sure you make ALL the OBs aware, you are going to get MUCH higher titers with gel than with tube... that said, I put a policy in place recently dealing with this. We identify antibodies in Solid Phase. Any AB's that may need to be titered must first be tested NEAT in the tube as you would your titer. So, our titer SOP states we use 3 drops plasma to 1 drop RC, incubate for 1 hours at 37C, wash and add Anti-IgG. If it reacts in this test 1+ or greater, the tech must titer. If weaker, then I report the titer result as Less than 1.
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Cerner class expert needed
yolis76 replied to mlodermeier's topic in Computer Systems / Software / ISBT128
We are always removing items from TMT. You need to go to TMT, choose the stay in which the antibody was originally added. You'll get a list of items added during that stay. Choose the line you want to delete. You can only do this if you know when the antibody was added, which is sometimes difficult to figure out. -
I have seen this happen a few times with patients... I think sometimes any trigger to the immune system will cause other previously formed antibodies to show up again, even if you gave Ag neg red cells. We currently have a case of a patient with a Hx of Anti-Fya, had a couple Fya neg units given at another facility, came to us a few days later with a positive DAT and Anti-Fya in the eluate.... according to the patient he did not get any other blood, and the facility insists was given Fya neg blood. I am constantly challenged to understand such strange occurrences, to the point that I get exhausted! Also, in regards to your re-XM of c neg E neg units, the Anti-Sda could have made you XM incmp.
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Thank you everyone for your replies! I don't think our washing technique is an issue, although it would make sense that if you manually wash an Rh pos with an Rh neg you could cross contaminate. We use cell washers for everything. I don't think the washer is the source of contamination either, because we have used 3 different ones, and tried washing manually, with the same results. Again, thank you everyone for your insightful replies!
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We have had more than a few instances in which the fetal screen is obviously positive on a maternal post delivery sample (NOT weak D), but the KB is reported as completely negative. Has anyone else seen this? What could cause this? I have worked with this Fetal Screen kit for about 6 years and I have only seen this in the last 2 years or so.