mondayw

Hi Exlimey, this makes sense. Thank you for taking the time to explain it thoroughly. I appreciate it! 

I tend to think that there are a number of factors affecting the reaction, such as changes to the pH, rather than just dilution, that would change the equilibrium constant within the Law of Mass Action that governs antibody/antigen reactions, but pH is only one of them.

I guess the solid phase is more sensitive to detect the auto in this patient. I will use the same method as detecting the antobodies to do crossmatch. Of course I will do saline and AHG crossmatch as well. Just my humble opinion.

Our SOP is to start with testing in gel (we use Vision Max) - if screen all pos, do panel, auto control and DAT.  if DAT is pos, do eluate - also tested on Vison Max.  if everything consistent with warm auto, we will then test patient plasma in LISS.  Often times the reaction is negative with LISS.  Apparently it has something to do with LISS and the sensitivity of the test......I don't know the exact science behind it, but,  if there are underlying clin. sig. ab's, they show in LISS where the warm auto does not.  If patient has been transfused in the last 120 days, we also will test the eluate in tube / LISS

Thank you for the warm welcome everyone! I’m a silent reader of this forum since this year and just decided to create account. I’m a generalist tech for over 6 years now but have started Blood Bank in 2022. I hope to continue learning from everyone’s experiences and thanks for making such an awesome forum where people working in BB could get insight and help each other out. 

Thank you for the warm welcome everyone! I’m a silent reader of this forum since this year and just decided to create account. I’m a generalist tech for over 6 years now but have started Blood Bank in 2022. I hope to continue learning from everyone’s experiences and thanks for making such an awesome forum where people working in BB could get insight and help each other out.