Bet'naSBB

Of course, my facility is blocking the link..... Is this a web page? Could you private message me the link please? TIA

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We will dilute the 3% to 0.8% and run in gel but with 40 min incubation. We found when we validated the process, it was imperative that the cells be diluted according to Ortho's "recipe" to dilute cells. While validating, we saw some false negatives when the dilution was "eyeballed" and/or when only incubated for 15 minutes. We might take to another media in certain cases, but PEG is our first choice....we will take to tube in a few situations...Patient on DARA, apparent antibody to gel itself or, antibody to preservative in gel cells (everything pos, auto neg). In cases of the last scenario, we will often try diluting a 3% screen from a different manufacturer and run those cells in gel to see if it's the cells, or the gel.......usually if the AC is neg, it's the cells. We are a large academic med ctr, and are the "reference" lab for other smaller sister hospitals so we do have the advantage of having access to screens and panels from multiple manufacturers.

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agree with @Neil Blumberg. We courier blood / platelets to our off site infusion centers on a daily basis and also to our small sister facilities that might need products. none of them have a separate FDA registration. We also send out WB, Plasma and RBC's on neighboring county EMS units and our in house Air units and none of them need FDA registration. Our medical director checked with his FDA "connection" and they sent an email stating as such.

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Anyone else having issues with opening the BBTalk daily emails since 1/1/25? They are coming to my inbox and then things come to a LONG pause and this is what I see...... I'm "older" and consider myself moderately tech savvy - but this is beyond my scope

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Anti-Lw? Don't know much about it - but I'm pretty sure it "looks" like a D - but then it's not................just googled and found this: when testing for anti-LW, a key step is to use enzyme-treated cells to confirm its presence by observing a reduced or absent reaction compared to untreated cells

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We follow virtually the same process as outlined above......... When we switched from tube to gel titers, we found that the most comparable results (if not performed by the instrument) were found when incubating the gel cards 40 min (ie - manual gel) that being said - we do perform at least 90% of our titers on the Visions.

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We are a Level 1 adult and Peds trauma center.....we don't have a limit............ We will give any MTP patient O POS (leuko reduced) LTWB until our supply runs out. We will give Oneg to peds - but if we run out of Oneg - they will get Opos. Our facility is currently involved in several studies using WB in the trauma setting. In the words of our Medical Director and manager..........."They have to live to have a problem" Might sound crass to many - but, it's true. For all the patient's we have transfused out of group WB to - we have had VERY FEW delayed reactions - maybe 2 anti-A's in the eluate and a few anti-D's - but all were males. Our 1st concern is saving the patient.........

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We only run QC if we are diluting an expired cell. (we run QC on any expired cell - whether we dilute or not) We performed a validation before beginning and validated that the dilution worked if incubated for 40 minutes which is the longest allowable time to incubate per the IFU.

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