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About Jermin

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    Biomedical Scientist (Haematology/Blood Transfusion)
  1. Rh+K Phenotype Validation

    Sounds like a very good idea indeed. Point taken. I should have also clarified that I was actually going to use the BioRad diluent (we just so used to calling everything PBS). Also I was not going to dilute it too much, so the concentration would be similar to a pRBC (how would I achieve such consistency? God knows). In the end it was just all a rough draft, which I can can assure you I will not be carrying out, and I am glad you have given me such pointers. I will contact the BioRad and see what they suggest. If they can't help much, I will use the donor units with known Rh phenotype.
  2. Rh+K Phenotype Validation

    Thanks for the replies. Thanks for the insight Malcolm, I always assumed that there was a lot of K- units on the donor units that did not have K antigen status indicated. I will use the donor units in that case, and see where I get. The DiaMed reagents are, but not the NBS reagents. Thanks for that question, as it slipped my mind. Yeah, I think 10 might be too small as well, but as I don't want to use excess cards, and I want to perform the test manually and two analysers, I can't see myself getting more than 10, but I will go back to the list of possible genotypes and review the number. In the end, there is no point in being frugal if it means that the whole validation process was not undertaken properly. I had it in my mind that validation is what the laboratory conducts, and verification is done by the manufacturer. Yeah, all Rh phenotypes are clearly indicated, but as for K antigen status, it is not always clear on the donor units. I will see if I can get that information from the the National Blood Service I wish I knew how I could do that. I will need to probably check with the manufacturer's reagent sheet and see what sort of limitations and discrepancies there might be, otherwise it might be beyond me. I was planning on getting the reagent cells, centrifuge them to separate from the preservative it is in, then resuspend it in PBS. But I might go ahead with using donor units, so this might not be an issue anymore
  3. Hi All, I am about to venture into performing my first ever validation of a test. I have been tasked to validate Rh+K phenotype testing on the BioRad IH-1000 (two of them). We have been, until now, performing the test manually, but as work is getting busier, performing it on the analyser might prove easier (or at least motivate people to perform phenotype). I have been given advise by my senior on how to go about it: Select 10 Donor Red Cell units which have Rh+K phenotype performed Perform the phenotype manually Perform the phenotype on the analyser compare the result pat myself on the back, provided I don't mess it up The issue I have is donor red cells doesn't indicate if they are K+, and I wanted some K+ as well as K-. I could always keep testing a lot of donor units until I come across a K+ unit, but I don't want to was a lot of cards (but that might be my last option). If I choose the NBS or BioRad Antibody Panel Cells, then the issue is the strength of the test cells, as they are, I think, 0.8%, and it does not fully represent the way we perform our phenotype manually, as it uses around 5%. I can always try and make the strength of the solution stronger, that is another option. So this is where I am at. If anyone has a suggestion, or better a complete plan, then I am happy to hear it. Also if there is any point I missed or need clarification, please feel free to ask, I'm fixed to this specific thread all night long. Cheers, Jermin
  4. BloodBankTalk:Allergic Reaction

    I just answered this question. My Score PASS  
  5. I just answered this question. My Score PASS  
  6. Phenotying

    A further question. I saw a senior BMS putting a manual tube grouping in the fridge for ABO typing. I know that there will probably have a better reaction in reverse group, but according to the senior BMS, the patient appears to have a weak D, and the BMS wanted to see if the reaction will be enhanced, but in forward grouping. I was confused since the books mentioned Rh bonding being hydrophobic and therefore warm-reacting. There was no enhanced reaction, but I was wondering if putting the test in the fridge would indeed cause an enhanced reaction for D, or be it on ABO, on forward grouping? Would it be due to IgM monoclonal antibody being used?
  7. Phenotying

    Thanks exlimey for a really good explanation, with background information. Always nice to put an answer in an easy to grasp wording. Cheers Malcom, gives me more confidence to seek out answers to questions (which I have plenty of)
  8. Phenotying

    Hi, This is a very silly question, but I think I have confused myself. Why is it that when Rh phenotyping, we do not require incubation step, but when looking for antibodies, we need incubation to detect Rh antibodies?
  9. Presence of H antigen

    Hi Malcom, Thanks again for your swift response, has certainly helped me. Came across auto-anti-HI almost a month ago, but I don't know if we performed the test properly to differentiate between auto-anti-H and auto-anti-HI, but it was the BB manager's decision, and as procedure doesn't change on how we treat both, I guess it doesn't matter. CheerS
  10. Hi All, I hope someone can help me with some clarification. I was reading about the presence of H antigens in different ABO blood group patients, but I am getting different answers depending on what I read. Transfusion Medicine and Hemostasis- Clinical and Laboratory Aspects- 2nd Edition mentions O > A2 > B > A2B > A1 > A1B with O having the most, whereas BBTS Introduction to Transfusion Practice-6th edition mentions O > A2 > A2B > B > A1 > A1B. Maybe it doesn't really have a big impact to my routine work, but it is still good to be in the know. Any thoughts would be welcome. Regards, Jermin
  11. Educating the Masses

    I saw a video recently, but I had a look at the comments. I guess we all become experts in biology when we use the internet. Has there been any 'facepalm' moments for you lot?
  12. M antigen and antibody

    Decided to revisit the post, just because I was trying to find some information of ABO IgG antibody causing agglutination. I have yet to see any literature which mentions this, so I was hoping you, or anyone else, can explain to me how this is possible? Is it a similar situation like M antigen being present in the extreme end of glycophorin A? Bonus point for explaining it to me like I am a 5-year-old
  13. Hi gagpinks, Thank you for your reply. I think I may need to clarify my queries I was referring to the guidelines set by BSH. We do not use a manufacturer supplied foetal spiked red cell reagent. We create our positive controls, and the reagents used for fixing and staining Kleiharuer films do not mention how to go about making the slide, just how to stain them. Again, I was referring to the BSH guidelines on storing controls, not maternal sample. I see what you mean. I will check the ISO manual and see what I can derive from that. Thank you, Jermin
  14. Hi Malcolm, As always I am always glad to see your replies. Thank you for educating me about the Rh blood group system. I have since read the BBTS chapter on Rh Blood group system and made some amendments to my specialist portfolio. I have always been good at being told what to do, but never thought to ask why, an issue which I aim to rectify. As to answering my FMH estimation query, I am again grateful for you comprehensive explanation. It has been beneficial, and I feel more prepared if I come across this scenario. Cheers, Jermin
  15. I was reading the BSH Guidelines for the Estimation of Fetomaternal Haemorrhage, and I had a few questions: 7.2 Slide Preparation In my current workplace, we do not dilute the sample, and my previous workplace did. I could not see a significant difference on the slide that was made and found them both equally adequate, but I was wondering if other have any input on them 7.3 Controls We currently prepare the control slide a week in advance and not fix or stain them, until required for batch testing. I have checked all the slides after it had undergone AE test, and all of them had been adequate. I was wondering if there is something which we are overlooking? These were the only things that were nibbling at the back of my mind after going through the guidelines. If someone could put it to rest, I will be grateful. Bonus Question I was wondering if transfusing Rhesus D Negative pregnant women have any implication on the FMH screening/ quantification, and what they would be? Cheers, Jermin