Requirements of A1，A2，B，O

[ccibp06]

I have noticed receiently that many reagent red cells for ABO reverse grouping ,like product of Alba,Meiton,Biotest(now Bio-rad),the
Rh phenotype is ccdee. Hope some one can explain why the Rh subtype is ccdee? is there a FDA requirement?
In east Asia,the RhD frequency is extremely low,it is very difficult to find such donor for the reagent manufacturing!
 

[Malcolm Needs ☆]

It is purely because anti-D is the most common alloantibody in the western world, and can sometimes (not very often) be detected by saline agglutination.  Therefore, this can give a false positive reaction in the reverse ABO grouping, which is dangerous.

 

However, like screening cells, antibody identification cells, etc, reverse grouping cells should be tailored to the ethnic group against which they will be tested (e.g. a Js(a+) cell should be included in a panel used against people of African ethnicity, a Ul(a+) cell should be included in a panel used against people of Scandinavian ethnicity, a Di(a+) cell should be included in a panel used against people of Chinese ethnicity, etc) and this extends to ABO reverse grouping reagent red cells.  So, if you are unlikely to see anti-D, because there are so few D Negative individuals in your native population, I see no reason why D Positive reverse cells should not be used.

[ccibp06]

Thank you very much, Malcolm. I totally agree with you that the reagent cells should be tailored to different ethnic group.  still I wonder why the FDA approved Reverse grouping cells are C Neg. and E Neg.? Is anti-C,anti-E also commonly fund and may cause direct agglitination in

serology practice?

[Malcolm Needs ☆]

Anti-C is quite rare as a monospecific antibody (it usually occurs with anti-D, and even then is usually actually an anti-Ce [Rh7], rather than a pure anti-C).

 

Anti-E, on the other hand, is extremely common as a monospecific antibody, and will quite often cause agglutination without the use of a potentiating agent.

[ccibp06]

Thank you,Malcolm!

[galvania]

The important thing is not WHAT phenotype they are - but that that is written on the label or wherever is appropriate if it is not consistent from lot to lot.

Edited July 2, 2015 by galvania