Posted April 13, 20187 yr comment_73082 I'm work in small town and sometimes we have problems to find some reagent like bovine albumin reagents In our blood bank we use manual cross match ( tube method ) My question is can I prepare the albumin reagent in the lab ? Or is there any method to reduce the incubation time for croos match ? Because when we don't have albumin we extend the incubation time to one hour. Or is there any website I can order it online
April 17, 20187 yr comment_73095 Who do you get your other reagents from...like anti-A, anti-B, etc.? I would assume that you would be able to order on line directly from the vendor/manufacturer of those reagents. Instead of albumin, I would recommend a switch to a low ionic strength solution (LISS, Lo-Ion, N-Hance are some of the name brands readily available in the US). Shorter incubation time and readily available from reagent suppliers.
April 18, 20187 yr comment_73111 I purchase a 22% albumin from Hemobioscience H305 (5X10ml). I dilute it and then use it to make up QC reagents. I don't know if they have different strengths or not. We use Bio-Rad's LISS reagent called MLB2 (1X50ml size) for routine tube testing.
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