The following article from Lab Medicine discusses the idea of routinely using enzyme treatment to enhance non-specific (we call them equivocal) antibody reactions.  The idea is that some of the reactions we end up calling non-specific (where remaining significant alloantibodys have been all ruled out) may be actualkly developing significant alloantibodys.

https://academic.oup.com/labmed/article-abstract/48/1/24/2666003/Ficin-Treated-Red-Cells-Help-Identify-Clinically?redirectedFrom=fulltext

Other than reference labs, does anyone routinely try to otherwise enhance reactions to see if a non-specific pattern turns into a specific one?

Thanks, Scott

We do.  Based on a poster at AABB Annual Meeting 2-4 years ago, can't remember exactly.