Yeast in screen cells

[LaraT23]

Was wondering if anyone else has had yeast growth contamination in their screen cells? We use Ortho surgiscreen, and had yeast growing in cell one only. It was this odd brownish color and was giving false pos results on almost every patient, so we cultured it. Low and behold we had a pure yeast growing in less than 12 hours. Any experience with this?

[lindam923]

Actually we have had trouble with Immucor cells becoming brownish. And yes there was yeast when we did a gram stain. I have had the techs place some drops of cells in a separate tube before entering with a pipette to see if it helps with contamination. We found that people were most likely contaminating vials when they went directly into the vial with a pipette.