lacs Posted June 7, 2011 Share Posted June 7, 2011 to run in parallel with EGA-treated cells when antigen typing red cells post EGA treatment as a control. I forgot the reason for doing this. Could someone remind me? I thought it's to check for polyagglutination when your treated cells expose to anti-T? but we're running this against manufactured antisera and not against patient serum (which has anti-T).... Link to comment Share on other sites More sharing options...
angelareflab Posted June 9, 2011 Share Posted June 9, 2011 Does the EGA product insert instruct you to run this control in parallel? It seems overkill as long as you have a single negative antigen typing result somewhere...and your post treatment DAT is negative Link to comment Share on other sites More sharing options...
lacs Posted June 10, 2011 Author Share Posted June 10, 2011 Thanks Angela. I think it's redundant too but it states in the technical manual. I think I'll just note to run that only if all Coombs results are positive, even if the DAT is negative. Link to comment Share on other sites More sharing options...
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