Patient cells A1 Rh positive with Anti-A1 in serum

[missyg]

Any ideas?!?!?!?!?

Cells were treated with ficin and DTT no change in reaction in cell ABO typing. Cold agglutinin is present with patient autocontrol postive at immediate spin, room temperature and 4C. DAT was weakly positive with polyspecific AHG and anti-C3. No reactivity at immediate spin or room temperature with 3 examples of A2 cells. Hopspital did compatibility with A1 positive cells and A1 negative cells and the A1 negative were compatible and the A1 positive cells were incompatible.

Thanks!

[Malcolm Needs ☆]

Just as a matter of interest, how did you A1 type the patient's red cells? Did you use Dolichos biflorus?

The reason I ask is that this lectin also contains an "anti-Cad".

I know that this is a bit "left field", but it could just possibly be that the patient's red cells are A2, with anti-A1, but are also strongly Sd(a+).

There I go, looking for zebras again!

On the other hand, auto-anti-A1 has only been reported very rarely.

:confused::confused:

[missyg]

Yes we use the Dolichos biflorus. We didn't even think about the anti-Cad, Sda possibility. We will explore that possibility!!!!!

Thanks!!!!

[Malcolm Needs ☆]

Yes we use the Dolichos biflorus. We didn't even think about the anti-Cad, Sda possibility. We will explore that possibility!!!!!

Thanks!!!!

Don't be too upset if that is NOT the answer; the theory is pretty left field, as I said.

At least auto-anti-A1 is rare, but recorded; my theory is not!!!!!!!!!!!!!!!!!!!!!!!!!

:D:D:D:D