ANTI-S,s

[irshadaad]

ANY IDEA FOR CONFIRMATION OF ANTI-S AND ANTI-s

[Malcolm Needs ☆]

I'm sorry irshadaad, but I'm not sure I fully understand your question.

You would confirm anti-S and anti-s in the same way that you would confirm any other antibody specificities; in this case using S+s- and S-s+ red cells by IAT to exclude other specificities, and S and s type the patient.

I know that various papers and books say that the S and s antigens are variably affected by papain (usually destroyed, but not always) but a paper by Jill Storrey et al a few years ago suggested that "papain-resistant anti-S" is almost always a low-grade auto-anti-U (or, at least, that's how I read it), and certainly, when we've bothered to actually test such antibodies in our lab, this is what we have found (a lot of work for not much return).

:confused:

Edited December 2, 2009 by Malcolm Needs

[irshadaad]

thank you,i read somthing about performing IAT @low temp .incubation instead of @37c what you want say about that

[Malcolm Needs ☆]

thank you,i read somthing about performing IAT @low temp .incubation instead of @37c what you want say about that

Well, it is true that there are a small number of patient anti-S samples, and slightly more anti-s samples found that have quite a high proportion of IgM to IgG, and so a lower incubation temperature may well help in identification, but I would recommend putting up two tests, one at 37oC and one at room temperature.

[GilTphoto]

Sounds like an anti-U to me. If the patient is black and S-s-