bacsyhieu Posted March 17, 2009 Share Posted March 17, 2009 Dear all!My blood donor is positive with HCV quick tests ( many times with many tests from supliers) but negative with ELISA screening. Does the blood unit that was collected from the blood donor use for transfusion? I get confused and want to know more information about this case. I hope to be shared from all.My very best wish.Hieu Link to comment Share on other sites More sharing options...
David Saikin Posted March 17, 2009 Share Posted March 17, 2009 I am not familiar with HCV quick tests . . . in the USA we only have the ELISA for HCV antibody (used for donor screening). What is your policy for this test result? If you have none, I would suggest one be developed. Link to comment Share on other sites More sharing options...
Abdulhameed Al-Attas Posted March 18, 2009 Share Posted March 18, 2009 (edited) The QUICK Tests usually lack SPECIFISITY,they reac with Any thing that is immunological similar but diagnostically different.It is better to prevent than to creat a medical error. Edited March 18, 2009 by Abdulhameed Al-Attas Link to comment Share on other sites More sharing options...
geekay Posted March 18, 2009 Share Posted March 18, 2009 Hi,Any rapid test by any manufacturer can give false positives, just like ELISA ! In addition, due to the lack of specificity , ELISA can be trusted more than any "rapid tests".. If I had been in your position, I would have gone ahead with NAT for the sake of the Donors interest ....and confirmation....and for the patients safety, I will never use this Donors blood for transfusion...( as per AABB protocol !) best wishes ! Link to comment Share on other sites More sharing options...
bacsyhieu Posted March 22, 2009 Author Share Posted March 22, 2009 Thanks for all ideas. I have checked that sample with NAT, and it was positive. Link to comment Share on other sites More sharing options...
adec1023 Posted April 27, 2009 Share Posted April 27, 2009 Why did u do rapid for the donor? dont u just go for ELISA only? Link to comment Share on other sites More sharing options...
transfusionpgi Posted May 26, 2009 Share Posted May 26, 2009 Thanks for all ideas. I have checked that sample with NAT, and it was positive.You see, engeekay was right, better to discard one unit than be in doubt. We too discard any sample coming positive by any (be it rapid, ELISA, etc.) technique. Also, which ELISA kit are you using? This type of result can come due to delay in tesing, sample error, sample storage conditions, technical error, using low sensitivy ELISA kits, etc. There are many good rapid tests available in the market which perform better than some ELISAs.Amongst all Ortho ELISA seems to be better than the most in the lot.Thanks for sharing the info. Link to comment Share on other sites More sharing options...
redstaff2003 Posted June 1, 2009 Share Posted June 1, 2009 hieu,in our situation before use of rapid test is common but right now EIA for screening test is best use. if initial result shows reactive we repeat test. if still reactive we usually refer it to reference laboratory for confirmatory. our algorithm says to repeat test in duplicate if still reactive we refer sample to a reference lab for confirmatory....by the way do you have any other screening test available at your lab or a back up test kit? Link to comment Share on other sites More sharing options...
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