unusual case

[sushi]

I am working on a antibody id case. It is a female with 3 miscarriages.

Her antibodies panel is all positive at PeG-IAT except the patient contol. Ficin-IAT are neg, DTT-PeG-IAT are all pos. Ch-, Rg- cells are still pos. 2 out of 3 Ge -2 cells are neg.

But the patient was typed as Ge2+. Further id, Ficin-PeG-IAT all came back pos except the pt. control. DAT neg tube and gel.

It is not Ena because she is M-N+

Also she is Vel+

Also anti-Tja ruled out.

Any one has any suggestion or ideas?

Please share. Thanks.

[N Mullis]

More information please! Do you know her ethnic background? What is her blood type? Were there any reactions at immediate spin? How strong is the reactivity? Since the enzyme-treated cells only reacted when tested with PEG, does that mean this antibody is non-reactive without enhancement media? What does it look like if tested by LISS 37/IAT, Gel or with no enhancement? Did you try to titrate it? Could underlying antibodies be interfering with your results? Will it adsorb and elute?

[sushi]

The patient is white. IS neg, RT neg, 4C positive with sc I,II, III. AC neg, Cord cells neg. Blood type group B/D positive.

PeG-IAT 2+, Ficin-IAT neg, DTT-PeG-IAT 2+. Anti-Ch/Rg ruled out.

Only 2 Ge:-2 panel cells are neg at PeG-IAT.

No LISS or Gel were tested.