New Blood Bank Technical Lead.

I have a lot of experience in gel but have recently started a new job in a facility that uses Solid Phase with the Echo Lumena. I recently reviewed our ABO policy and it states that any Rh negative result for a patient without history must be confirmed by tube before resulting. Is this a recommendation from Immucor? I can't find much about the limitations of the CMT plates. 

It would also be a huge help if anyone was able to point me in the direction of references for Solid Phase ABID troubleshooting? or is anyone willing to let me view ABID Echo Lumena troubleshooting job aids so I can get a feel for that before reviewing/editing an Antibody ID policy? 

Thank you for any and all help. 

Edited March 15, 20241 yr by Lindsey01

Hello! I've worked with Immucor over my entire 18 years in the BB - you should be able to reach out to your account rep for the info you are seeking regarding ABID troubleshooting. They have a collection of white papers that can help you. 

Regarding your Rh negative confirmation in tube - that sounds like something specific to your facility. It's not an Immucor recommendation that I am aware of, nor do we do that at my facility. The CMT plates are literally just a blank microtiter well, so the reactions are very similar to tube, and are read the same way. The trouble with the instrument reading the reactions is that it will not detect mixed field or rouleaux and can have false positives because of that. 

I am new to a machine (echo lumina) that constantly give equivocal (?) readings.  This seems to make ABID reading harder. Have to go to tube here ( repeat screen). Then if positive go back to the machine we already were steered away from to run ready id. This makes no sense.  So is this particular machine not calibrated in reactions sufficiently ?   Is it normal to keep jumping methods to get around the ? Readings which in LISS are always negative.