Blood_Banker 13 Posted July 31 (edited) There is a donor that reacts with two polyclonal anti-s antisera from 2 different manufacturers but not a monoclonal anti-s (contains clone P3BER). Is this likely to be an s antigen variant? Quite sure that the donor was genotyped as s positive which is the only reason we tested with polyclonal antisera after we got a negative result with the monoclonal anti-s. Donor is Mi(a+). Anybody seen anything similar? Thanks in advance. Edited July 31 by Blood_Banker Share this post Link to post Share on other sites
exlimey 342 Posted July 31 Yes. In the examples I've seen, the usual the culprit is a gene re-arrangement that results in expression of the Dantu antigen. If I remember correctly, the P3BER clone does not react with Dantu+ cells. If it isn't mentioned in the Directions for Use, you could check with the technical people at Millipore/Bioscot. The presence of "Mia" (an obsolete umbrella term that can apply several "Miltenberger" antigens), already indicates that some MNS gene shuffling has occurred. 1 Blood_Banker reacted to this Share this post Link to post Share on other sites
Baby Banker 75 Posted July 31 2 hours ago, exlimey said: Yes. In the examples I've seen, the usual the culprit is a gene re-arrangement that results in expression of the Dantu antigen. If I remember correctly, the P3BER clone does not react with Dantu+ cells. If it isn't mentioned in the Directions for Use, you could check with the technical people at Millipore/Bioscot. The presence of "Mia" (an obsolete umbrella term that can apply several "Miltenberger" antigens), already indicates that some MNS gene shuffling has occurred. I was going to suggest that you check with the manufacturer. If you haven't already, look at the package insert. 1 Blood_Banker reacted to this Share this post Link to post Share on other sites
Blood_Banker 13 Posted July 31 Thanks. Finally found a great journal article about this phenomena - just takes googling the right keywords to find something. Here is a link for those interested. https://onlinelibrary.wiley.com/doi/full/10.1111/vox.12909 1 1 exlimey and JasonS reacted to this Share this post Link to post Share on other sites
exlimey 342 Posted Monday at 10:55 AM On 7/31/2020 at 4:42 PM, Blood_Banker said: Thanks. Finally found a great journal article about this phenomena - just takes googling the right keywords to find something. Here is a link for those interested. https://onlinelibrary.wiley.com/doi/full/10.1111/vox.12909 Oops. Perhaps "Mia" is not as obsolete as I believed. Great article/reference. Share this post Link to post Share on other sites
e specificity 3 Posted Monday at 12:58 PM We had a situation with discordant/variable little s typings (but caucasian) and genotyping came back without flags as little s+. In the end, the patient had a glycophorin hybrid that required sequencing to determine with an antibody classified as anti-Ena. Share this post Link to post Share on other sites
e specificity 3 Posted Monday at 01:04 PM To add, Reid's The blood Group Antigen FactsBook 3rd ed. MNS system pages has a nice breakdown of the "Miltenberger" phenotypes for the hybrids. Share this post Link to post Share on other sites
exlimey 342 Posted Monday at 01:19 PM 18 minutes ago, e specificity said: We had a situation with discordant/variable little s typings (but caucasian) and genotyping came back without flags as little s+. In the end, the patient had a glycophorin hybrid that required sequencing to determine with an antibody classified as anti-Ena. Eek ! I hope they don't need transfusion. Share this post Link to post Share on other sites
