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Showing results for tags 'crossmatching'.
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Hi All Historical A POS (never phenotyped) no antibodies detected. MDS patient, multi-transfused with APOS/NEG units over past 3 months. Results Screening cells (using BioRAD 3 cells) +2 positive reaction only with R2R2 (cell II). DAT +2 (IAT method/ IgG/C3d polyspecific) Panel negative which suggested a low prevalence/frequency/incidence antibody however - crossmatch was positive which led the question was there was another underlying antibody ( a soup of antibodies). Reference Lab Results Possible Jka by IAT enzyme and non specific enzyme reactions. Now awaiting genotyping. Advise to crossmatch Rh/K/Jka D-C-E-Jka- My question is now the patient has returned to group A POS (there was a dual population before with the D type due to the previous transfusions) is it acceptable to transfuse D+ (as historically RhD+) C-E-Jka- units or could there be a potential D typing anomaly ? Thanks
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In South Africa it is legislated that the ABO/Rh be confirmed on the segment from the pack before transfusion. When the blood bank changed to an automated crossmatch method, segment testing remained manual due to the cost of automated grouping. The cost of testing the ABO/Rh on the Olympus PK7300 proved to be significantly lower than other methods like Autovue etc. While there were substantial benefits new problems emerged. The tediousness of the task of cutting the segments manually to decant the blood into the test tubes (800-1000 per staff member), as well as manually comparing the bar-code numbers. We have now developed an automated segment cutter. The segment cutter prints the bar-code on the test tube, cuts the segments, decants the blood and adds saline. The racks with test tubes can now be placed directly onto the Olympus for analyzing. The decanter can process up to 300 segments per hour and sends the information to the LIS. Now my questions are the following: • Are the cross matching processes the same globally, i.e. cross match on segment not on specimen? • How common is it that the blood banks do all testing in bulk and send final product to hospital? I apologize in advance if I have some of the terminology wrong. I designed the machine and my blood testing knowledge is limited. Any information would be greatly appreciated. Thanks in advance.
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- crossmatching
- segment decanter
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