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Showing results for tags 'false positive'.
Found 3 results
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Our facility is evaluating making a change to our process for Weak D testing for patients with a positive DAT. For years, if we were required to do a Weak D, but the patient had a positive DAT, we used to cancel the Weak D as invalid. Another hospital in our system mentioned that they tended to perform the Weak D, but then only cancel as invalid if the Weak D is positive. We are thinking about changing to this process, as we now have to result many babies as "Rh Unknown" and give their mothers Rhogam. Per our Anti-D's package insert: "Red blood cells coated with alloantibodies or autoantibodies of the same or similar specificity as the reagent (i.e. cells that are DAT positive) may give weak reactions. This is due to decreased availability of antigen sites because of antigen blocking or steric hinderance. In extreme cases false-negative results may occur." I'm worried about the "extreme cases" where a false negative could occur, but I cannot see this being common. Also, would you think that if the cells were coated with that much antibody, that we would see any other odd reactivity in the ABO/Rh? What do other facilities do? Thank you in advance, Susan
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Hi. We encountered an interesting case tonight. A male patient with no history of transfusion (also said he's never been transfused) typed beautifully A Pos. His screen, however, was positive 3+ for cells 1 and 2 and then D pos panel cells ranged from 1+ to 3+. The D negative panel was negative and antibody ID panel was positive for all D pos cells. We then looked as his med list. He's on Bicalutamide, but there's no mention of BB interference in any literature I've Googled. He has a hx of prostate cancer and family hx of malignant neoplasms. Tube results were negative across the board (I used both LISS and no LISS to see if any difference). They were text book, beautifully negative at that for all phases. DAT negative. What could be an explanation of this??? Is there any cases of solid phase D reacting due to meds? Meds are the only conclusion we've come to so far. Any thoughts??? -
Hi I am reference coordinator at 450 bed Level 1 Trauma Center. The last few months I have seen a few times when all three Neo screening cells are about 2-4+ pos and then Neo panel was all negative. These patients did not have Rhogam and tested negative in PEG testing. I have also seen this happen with several different Neo screening cells lots. One patient screen on Lot R369 was 3+ pos for all three cell on both Neo instrument but had a negative Neo panel on Lot ID201. Then this patient was tested against another screening Lot (R374). Which resulted in 3+ reactions on all three cell on one instrument but the screen was negative on the other instrument. 30 min Peg screen and auto control was negative. Immucor is reviewing this one but has not responded yet. Another patient example is a patient screen on Lot R384 tested 3+ on cell 1, 2+ on cell 2, and ? on cell 3. Since the other Neo was down the Tech repeated the screen with Manual Solid phase and all 3 screening cells were 3+ pos. The Neo Panel DN072 was all negative and so was PEG screen and panel. Has any one else had a solid phase panagglutinin positive screen and corresponding neg solid phase panel?
