gagpinks

Thanks Malcolm. If auto and DAT are negative I would always think of HFA.

Hi Amy Feel free to ask questions. This is very good sites to share BT knowledge. The best person for this topic is Malcolm Needs as you can see in threads. Good luck

Just wondering due to patients phenotype, could it be Anti-f?

That's what my concern too. Another question how does anti f works? Does is react with LISS tube IAT?

A blood sample from an antenatal clinic (9 weeks gestation) showed 3+ reactions in the antibody screen. Antibody identification show 2+ reactivity in IAT and 4+ reactivity in enzyme techniques, with a negative autocontrol. The patient's phenotype is C+c+E+e+K-. The sample was sent to a reference lab, which reported non-specific reactions in IAT and enzyme , with no antibodies detected by LISS . DAT: Negative Any explanation for this results why strong reaction in screening and antibody identification but no alloantbodies

We have Received sample from Antenatal for G/S and found patient has Anti Yta confirmed by reference lab. I know Anti Yta doesn't cause HDFN however in terms for providing blood do we need Yta beg blood? Does this causes HTR if not provided Yta neg blood in an emergency?

Agreed. We are going to implement safe trace but there's many issues arise during configuration . I don't think so safe trace is really great system

How is your experience with safe trace? Is safetrace has good audit trail?

I guess patient might have multiple transfusion c+ in home country where they don't perform Rh phenotype.

How do you deal with this situation at your end?

Patient blood group is A Rh D positive and antibody screen negative

Hi We Received URGENT request for 1 unit of blood transfusion for 1 year old child who had previous transfusion at abroad due Thalassemia Major. This was patient 1st visit in the UK therefore Rh and K phenotype was not known . As per guidelines Rh and K phenotype performed. Rh phenotype results obtained as below C mf E 4+ c+4 e4+ K mf. (Probably R2r) Because patients was previously transfused at other country sample was sent for genotype which will take 2 weeks to get results back. Due to clinical condition, patient, required urgent transfusion therfore with Haematology consultation 1 unit of blood C-K- blood issued by IAT xmatch. Meanwhile sample was sent for Rh genotype and results received as C+E-c-e+ (R1R1). Totally different phenotype result Why? I know patient had multiple transfusion at abroad. But never seen. Thankfully we had consultant approval to issue C-K- blood. Lesson learned always involve clinical team to decide. Any further comments

We also inculed documents owner documents authorised by and effective date.

I agree also agree MU is not really for blood group and antibody screen . No clinicians will ask blood transfusion department how unsure are you for your blood group. it's just tick box excersise yo keep UKAS happy. We do MU for manual group and antibody screen and Rh and Kell phenotype. We take 10 sample of QC and equipment involved in this process. Calculate MU for equipment involved ( provided in calibration certificate)such as pipettes,Timer incubator and centrifuge and use calculations to count MU.

frm5197-41-fetal-rhd-screen-request-form-printable-version.pdf Why does this form says ( top of the form) if patients has Allo antiD sample will be rejected for Fetal RhD screening. So I am sure they will be using different technologies

Thanks Jayinsat It was really helpful.

What are differences between ISO 15189 (2012 ) and 2022 standards?

Yes. Another thought do you think in 1st pregnancy anti-D could be in IgM nature and therefore level might be slightly raised?

Thanks Malcolm. I checked it she was not given large dose of anti -D in her first pregnancy. However in her first pregnancy she developed anti D at 32 weeks where her level was 0.5Iu/ml then at end of her first pregnancy level was 1.0IU/ml. As per routine antenatal sample if booking blood is Rh negative we send sample for FDS for Rh D prediction. Could it be because lady had Allo antiD? When lady has Allo anti D do they use different techniques?

Hello Patient has developed antiD in first pregnancy at around 32 weeks and her quantification level was 1.0 IU/ml. In 2nd pregnancy her booking blood at (12 weeks) antibody screening was negative. At 15 weeks sample sent for fetal genotype (FDS). On this Report received inconclusive due to all Anti D. Because patients was on file for historical antibody therefore sample sent for quantification in 2nd pregnancy and Report received antibody not quantified since it reacted weakly in enzyme IAT only. My understanding standing is if patients once developed Allo antiD her titre level does not go down. Why was her antibody screen was negative in 2nd pregnancy at 12 weeks?

Hi Would like to know more about transfusion practitioner role. I am aware of main roles of TP are PBM, Education,audit and adverse events/incident management. Would like to know how can we modernised current TP role? Any tips from around the world would appreciate Thanks

Patient is not sickle or Thal, however Patient is child bearing age. Do you think should RCI have to investigated more?

Agree with you. No further samples were requested nor followed up Bit disappointed. patient was transfusion dependent and doing well.

Interesting case 3 units were crossmatch electronically. After 1st unit patient developed chills,rigor and Temperature, therefore transfusion was stopped and requested for transfusion investigation requested. upon investigation it was found pre and post transfusion Antibody screen and identification Panel was also performed and negative on both sample. DAT was negative on pre transfusion sample and positive in post transfusion sample. Ig G 1+ C3d negative. However crossmatch was performed by IAT and found to be positive 3+ reaction. Pre and post Samples were sent to RCI for investigation. Patien Billirubin and LDH went up significantly. Case was discussed with both consultants immediately. Reference lab found same result as lab result. They also tested donor unit for some low frequency antigen such as Wra, Doa Dob , Coa Lua Kpa and found negative. Unfortunately due to unavailability of rare reagent RCI unable to solve the case. Patient was excluded from electronic issues.

Pre delivery sample going for urgent c-section.