Hello, Sorry for my English, it is very basic but I will try to explain you my doubts. In my country (Chile) the crossmatch in the blood banks always has been tested in gel column. The glass beads column just its entering as technology, it is therefore natural we have some apprehensions, which I would like to be able to consult you. We have always worked the crossmatch with tube Red taken to patient (sample) in order not to discard the hemolysis as sign of incompatibility. INNOVA suggests will work with EDTA tube, according to what I understand this anticoagulant chelates Ca ++ and Mg +, therefore eliminating the action of complement. Does that then happens with those hemolytic antibodies, example anti Le, are masking a kind of incompatibility? It has clinical significance? Thank you and I hope you can answer me, greetings from Chile