Elizabeth Corbisez

For the past several years, as required by CAP, our facility does a semiannual method comparison. We run 3 positive and 3 negative antibody screens with the following techniques: ProVue, manual gel, Peg, LISS, and enhancement free (better known as saline). Gel is our primary method so all of the positive reactions were initially discovered in gel. We have seen excellent correlation in antibody detection with all methods although the strength of reactivity is admittedly weaker in LISS and saline. On one occasion, there was an antibody that was not detected in the saline technique, but was detected with the other 4 methods.