mkeepes

From what I understand if the button is too wet, increase the decant speed and if the cells stream up the side of the tube, decrease the decant speed. Good luck!

I think the pictures of the tubes look as expected after centrifugation and after decanting. The cell button won't be a tight button after decanting and there should be a small amount of saline present with it. I am setting up our first Helmer cell washer (always used IEC Centra W or Sorvall before) and have played with a lot of settings to see what worked best for us and I ended up back at the recommended settings (on the blue quick reference guide) for almost everything except I added a few more shakes (agitation) between washes because the cell button wasn't completely resuspended in a few tubes after addition of saline prior to centrifugation during wash cycle. I have our decant speed set to 435. I think the default is 440.

You might check to see if the "global setting" for Spin Decant M is on or off. I think it needs to be off. To get to the global settings press the left arrow until they appear and then press the left arrow to toggle through all of the settings, when finished press Start Wash to save or exit.