keebie81

I turned the decant speed down quite a bit and seem to be getting a decent result. At the end i have about 1 drop of saline in the tube Is this an acceptable amount? The decant is at 368rpm

Here is an attached image of after spin and after decant

Thanks, it was to on. It seems to be doing better right now. After the spin step i can see a button and it looks good to me. But still after the decant the button breaks apart and looks runny. I will try attaching an image in a bit.

Yes i checked that, measured 56.4mL. This is the same value shown on display. I also measured the volume of the 12x75mm tube, it was around 4.5mL.

Ive gone through a few different saline cubes, the lot numbers have been different. The tubes are 12x75mm and filled with 4.7ml saline The wash(rpm) is set to 3500, Twash 0:35, decant(rpm) 425

I am using a SP Certified Blood Bank Saline cube from Cardinal Health

It is spinning, the rpms are all good. It seems to have a cell button but during the decant its gets lost. If i put the tubes in a centrifuge after a button forms

Forgive me if this has been covered already. I tried looking and did not see it anywhere. I work in the biomed dept and got a work order for the cell washer washing away all the cells. We have a Helmer UltraCW. I have spoke with tech support and we have gone over all the settings and they are correct. But it still washes away the cells, they even sent me a loaner machine and with same settings i get the same results. We are using Panocell 2-4% Reagent Red Blood Cells for testing. The way lab showed me to test is to put a drop in each of the 6 tubes, run the cycle and see if there is a cell button formed at the bottom. Everytime I do this I end up with nothing. But I have noticed if I press the check button after each step and look there is a button cell at the end of the 4cycles. I am looking for how other labs use this device for testing. Maybe our method is wrong. The operator manual isnt much help except for setting the machine up.