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Pre-heating the strips in the incubator for 20 minutes is not included in the manufacturer's directions, however, we got around that by doing an In-house validation of our own with strong and weak cold reactions that also gave us negative reactions with PeG. We re-correlate these reactions 2 times a year using a prewarmed techinque in GEL Cards, PeG, and the pre-warmed Echo strips. We use 12-20 specimens for the correlation.
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Usually all positive reactions on the ECHO and negative in PeG & LISS are due to a warm auto or drugs. We place a canned text comment on the patients specimen that states, " PeG antibody screen negative, no antibody identified." We follow up with a complete crossmatch. It can also be resolved using the Manual ECHO workstation. We pre-heat the antibody screen strips (Capture RS-3) in the incubator for 20 min., that will also resolve the problem.