dotahill

We do not have gel or capture yet. We do use the cells that are bracketted, but it still isn't enough. I was thinking about getting PeG becuase this is getting a bit crazy. If the doctor's would stop ordering type and screens 5 days after giving rhogam, it would be a big help!!!

Help, we are using a 16 cell panel and we can't rule out Anti-C or E when an Anti-D is present due to a rhogham injection! We are having to send our work-ups to the ARC for an Anti-D!! grrrrr. We ran 1 full panel and then 4 additional panels were used for selected cells and we still couldn't rule them out! Does anyone have any suggestions or noticed how the panels are starting to not be helpful at all!!??

We are having such a hard time in our lab right now ruling out Anti-C and Anti-E with our 16 cell panel when the antibody is an anti-D. We are still doing tube testing. I was wondering if anyone has run into this and what you are doing to combat this. I am getting sick of sending samples to the ARC for an Anti-D. I am trying to figure out best practice, i.e. bringing in a 12 cell panel in addition to our 16 cell, or bringing in PEG. The panels are starting to be very unhelpful!

Hi All, I am trying to buy a new cellwasher and wanted to know if anyone has one they love. We have an old Sorvall 2 which we love, but it is getting old and needs attention. We purchased the Sorvall 2 plus and did not like it at all. It left too much saline behind:( Let me know if you get a chance. Thanks

So I was flipping through AABB for fun and they say you can use 4 drops of serum to 1 drop of cells, if the proportion of LISS is kept the same. I still don't know where I am going to go yet with this issue.

We recently had this problem with 2 pregnant ladies at our hospital. To prevent this happening in the future, we elimanated weak D testing on all patients, excluding newborns and babies. Weak D patients are supposed to be treated as Rh negative patients, i.e. with rhogam and Rh negative products. This elimenates the need to explaining changing blood types!

For some reason I didn't see the last part of your statement yesterday. Duh. Thanks.

We are looking into gel and hoping to get it in soon. We always perform a cold screen with our prewarm. But I want to do away with the prewarm and use REst instead. Do you use PEG? We are a small lab that only keeps LISS and albumin on hand.

She was using 4 drops of serum and 2 drops of LISS. I have talked to her and explained that can't happen. Thanks for the post.

If you use it on occasion, do you use REST instead?

We are having a major battle at my hospital right now. The "old techs" as they called themselves are putting up a fight because I want to do away with our prewarm procedure becuase there is no enhancement media. I was wondering what other institutions are doing. It all started because one tech used 4 drops of serum to preform an IAT. Turns out this is normal for her as she doesn't want to miss an antibody. Package insert for LISS states the amount of serum to LISS needs to be in proportion to each other. Long story short, does anyone out there still use the prewarm procedure without enhancement media? Thanks.

We recently had this issue with 2 OB patients. Talk about frustrating. Both typed as Rh negative before pregnancy, and for their prenatal work-up, they were DU positive. These results led our lab to change our DU testing practices. We now only do DU's on cord bloods and babies. AABB supports that if a mother is testing DU positive, they should be treated as Rh negative and should receive Rhogam.

Hi all, My lab is looking to getting gel, any suggestions or horror stories? Thanks