Desoki

Thaks Malkolm But for another case when I should conceder anti M clinically nonsignificant-if its reaction disappear at 37 or what?

yes I did all tests after prewarming also I use Tube method for IAT not gel method

Dear Friends I know the clinically significant antibody; that's antibody react at 37c or react with AHG, but since one week I had a case positive antibody screen with immediate spine by tube method but still positive in all stages 37c and AHG, the antibody was anti M with negative autocontrol this negative was through all stages. My question is, shall I conceder this anti M clinically significant antibody because it still gives positive in 37 and AHG so give patient M negative unit, or I have to conceder this anti M clinically insignificant because that antibody react at room temperature and that continuing reaction at 37 & AHG is due to strong IgM not faded by incubation stage of indirect comb test, so no need to give M negative unit?

Dear colleagues, I want to know how many days' cells still valid after manual preparation of combs' check cells by isotonic saline method?

Dear friends when we should use albunim 6% as a control, and albumin 22% as control, and why?

Dear all I am confused, Please give me clear answer, when patient need platelets transfusion but we don’t have identical platelet group (same platelet group) for transfusion, what shall I consider in transfusion of platelets, antibody (even high titer or not high) in donor plasma against recipient RBCs or recipient Platelets, or consider antibody in recipient plasma against donor Platelets? Thanks

Today I had a strange case, need you scientific explanation: That case was for pregnant female 30 years old age A+ve, since two months she got two transfusions last one since one week, also she didn’t suffer from any thing other than anemia, only medications she took was Zantac and Palsil, her antibody screen was before today negative and all unites were compatible, and these transfusions done smoothly. Yesterday her doctor asked for one unit PRBCs, the technician did antibody screen and cross match by gel cards, results were negative but the patient has severe reaction after transfused with that unit, the reaction interpreted by blood bank observer as febrile nonhemolytic transfusion reaction. Today her doctor again asking one PRBC unit, but when we start working for this patient, her antibody screen became positive by gel card in AHG phase with three screening cell grade 3, autocontrol and DAT negative, we did panel with extended six cells the result was all cells positive but with different grades, we also did enzyme panel to save our time, the result was only two cells positive and interpreted as alloanti E. Also I wanted to repeat our work with manual tube methods, unfortunately the result of antibody screen with three cells were negative macroscopically but few mixed field under microscope and positive after adding check cells, also we did manual crossmatch, the result was negative of one unit macroscopically and microscopically and also strong positive with check cells so we released this unit, the second unit was negative macroscopically but very few mixed field under microscope and positive after adding check cells but we didn’t issue this unit. My question is why there is strong reaction with card nearly negative with tube? Also, is her drugs played a roll in this scene?

Thanks malcolm but which best cell to be used to anti D titeration (R1R1 which represent the the phenotype of 75% of asian like Saudi arbia where I work, or R2R2 which represent more antigen epitopes)

Dear colleague; I have 3 questions regard to anti D titration: - We already use saline manual tube method for anti D titration without any enhancement media (albumin or Liss), my question is if I add enhancement media to the test like albumin or Liss it will affect the result? Also shall I use coombs liss gel card in anti D titration or this may affect my result? I always selected cell R1R1 for this titration but due to long interval between titration and next one, we use also cell R1R1 but from different panel, is this will affect my result?

Dear colleague, Sometimes I can't get data from reverse grouping due to cold autoantibody; my question is if I use DTT or AET to unbound the disulfide bond of IgM molecule of cold autoantibody, it also affect anti A and anti B? In other words, is this method suitable for reverse grouping??

Dear colleagues Our blood bank received blood bags (double, triple and quadruple with filter) from JMS Company for evaluation, but we have no specific criteria for this evaluation. Some colleagues suggested doing normal QC for blood products (cuter, platelets counts, PH. etc…) also inspection for air inside bags and others. But I don’t have any paper or written documented criteria for this evaluation. If you please guide me where I find these criteria or put here any paper about this evaluation. Thanks for all

Did you wash the cells before tube method?

what about D control tube???

Yes Anna,I already considered homozygot cells, also I know if I try with another gel panel may success, but this not my question, my question was about why all results were negative with tube however it gives positive with gel?

No, I didn't check under microscope, I just comfirmed by CCC

yes Malcolm but may be I heard wrong from my collague, I asked her again now but this patient didn't receive blood since last two years

Two days before, I had a case referred from one colleague, this case was for female patient with sickle cell anemia age 18 year with previous transfusion, now patient is positive antibody screen with three cells of Diamed gel cards and when I did panel identification by Diamed gel cards the result was 8 cells of 11 panel cells were positive with different strength and autocontrol positive and DAT positive, but these positive cell didn’t lead me to any result so I tried to use another panel but only available other panel was for Biotest company but not gel, tube, unfortunately when I read the result of tube panel all cells became negative, tube antibody screen negative even tube autocontrol negative also crossmatch with units by tube also negative I comfirmed all the result s by coombs check cells, all negative give positive with CCC. My explanation is patient has something against gel or Liss in gell cards……….!!!!!!!!!! Do you have any explanation?

many thanks for aakupaku

Anyone can help me to solve this problem by send me isert of WARM reagent...!!!! my email is msdesoki@yahoo.com Thanks my friends

I know ZZAP but i didn't know other name WARM

One friend told me he heard about commercial Kit can remove warm autoantibody from the mixture leaving the plasma free from autoantibody so can test for possible alloantibody, Is it true and if true how that kit works?

I didn't react this sample with enzyme treated cells, but If I treat that sample with enzymed panel and result give me negative is this prove that Ab nonclinicaly significant?

I had a case of sickle cell anemia patient 20 years old female with recurrent blood transfusion last transfusion since 2 months, group A+, Ab screen positive with all three cells by IAT only with AHG not in immediate spine or 37c and positive with all cells in panel only with AHG step, also autocontrol positive and DAT positive. I suggest that warm autoantibody but I can’t identify it, so I don’t know how can I select the allogenic cell for alloadsorption or how can I remove this warm autoantibody without disturb the possible alloantibody which is more important than that warm autoatibody? Thanks for help

\Can the sample give any positive result to panel cell although negative with three screen cell?