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Everything posted by Joseph78
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Hi Ruth! Rare reagents may be used beyond their expiration date if appropriate positive and negative controls are run each day of use and react as expected. This is permitted by FDA. However, this does not apply to reagents that are readily available. Your laboratory should establish criteria defining which reagents are considered "rare". I got this from the College of American Pathologists. Joseph78
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We still use "least incompatible" but I agree with Malcolm (suitable for - is more appropriate). If we have patient with warm auto, we have the physician sign a consent but we put a comment: "The patient has warm autoantibody and all crossmatches will be incompatible. I request the least incompatible packed RBC's (NOTE: Units are crossmatch compatible with autoadsorbed serum at reference lab)."
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Hi Lara! I ordered the fetal screen from Ortho and it will get here next week. Did you do validation? If so, do you know I could order a validation kit? If not, what did you use for validation? Can you manufacture your own specimen? Thanks in advance.
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Hi! It depends... our policy states that if patient has no ABO history and is non-group O, we look for previously drawn sample preferably collected at a different time and by a different phlebotomist (there is no restriction as to when the sample is collected). If there is no previous specimen and blood is not needed immediately, we draw the patient. If blood is needed ASAP, we set up "O" Rh-compatible. We just started this last November 2009 and we only had one patient drawn for a 2nd independent sample.
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I concur. Are you planning to switch to other reagent kits? I hope that they will resolve this problem so I won't waste my time doing the validation.
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Hi! Do you have the recipes for manufacturing samples for FMH? I already ordered the reagent from Ortho and I will start my validation next week. I have already 3 positive samples though.
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Hi! I'm having problems with the Immucor Fetal Bleed Screen kit (I'm sure there's a lot of labs out there that has this problem). I was advised by Immucor to use buffered saline (pH 6.9-7.2). We are using buffered saline since I started working in this lab. I had two false positives last year that were K-B stain negative. The recent CAP survey for HBF reported that 30% of the participants reported it as "positive" (intended response = negative as specified by the vendor). 30% of false positive is not negligible. I'm planning to order reagents from Ortho. Can anyone share on where to order validation kit for FMH? I need 10 positives and 10 negatives. Thanks in advance.
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I had this experience a few months ago... wherein the ED doc ordered for an emergency release of "O" pos packed cells. We released "O" negative packed cells per protocol BUT the ED doc returned the units and insisted of getting an "O" pos. Luckily, the patient was "O" pos. The ED doc told me why question his request. I asked our Laboratory Director to talk to that ED doc to follow protocol since we are under strict regulation and unless the patient was already typed for the current stay then we can give type-specific regardless of previous ABORh history.