[Hla analysis system]

Dear Caricami,

Thanks very much for your reply. I am actually looking at the hardware required. ie, the equipments for doing the HLA. Could you please help me with the details used at your institute.

Regards

Manoj

There is not a single system for HLA matching.

For transplants, there usually needs to be at least a 4 of 6 match. The DR, DQ, A, B, C, and DP regions need to be tested. DR, DQ are Class II which are mostly what I deal with so that is where most of my knowledge lies.

For DR, high-resolution testing is needed, meaning that when all the analysis is complete, allele-level results will be obtained. First the general testing is done, which will identify the major probes that the specimen has (DRB1*01, DRB1*07, etc). Depending on the major probes involved, further subtype processing will need to happen, and possibly even secondary testing.

Once those results have been obtained, usually by sequence-specific oligonucleotide probing, the sample might be at allele-level by this stage. Otherwise, no further processing on the SSOP side can occur and the specimen will need to be reflexed to either SBT (sequence-based typing) or SSP (sequence specific priming). Between these results and the SSOP results, allele-level results have usually been obtained and a match/no match can be determined.

[Hla analysis system]

Dear All,

Could any one tell me if there is any single system for HLA matching? Or at least let me know what would be the procedure followed for HLA matching. This would be for Stem Cell transplants.

Thanks in Advance.

Manoj

[planning to get new equipment for blood bank in india]

Dear Dr,

I am a dealer based in Kochi, Kerala doing Blood bank equipments for the last 15 yrs. My recommendations would be as below. If need I can put you in touch with the suppliers in Puthuchery/ Chennai or even supply and install at your institute. The below equipments are used by many of my customers over years.

apheresis - Caridian BCT - Cobe Spectra (Stem cell, Platelet, WBC), Cobe Trima (Platelet, TPE)

stem cell storage equipment - Thermo Scientific Cryomed, Cryoplus Systems

cord blood collection and storage system - Storage systems from Thermo Scientific, Cord blood collection systemd from MACO pahrma.

leuco reduction system - Terumo Penpol TACE II +

sterile connecting device - Terumo TSCD

a walk in cooler / deep freezer _ Deep Freezers from Thermo Revco, you have volumes til 888 ltrs

Hope this will be of help to you

Regards

MANOJ

[Donor collection couches]

Is your requirement still live?

[Blood bank refrigerated centrifuge]

I have experience with the Hettich Rotosilenta. This is a good machine. Make sure you have Service rep available at your place.

[Central Temperature/Alarm System]

Dear Sir,

Try getting in touch with Oceasoft, France. They have a wireless system which can be upgraded for use with more equipments in future also.

http://www.oceasoft.com/uk/

[Vote for BBT's new name / address]

If possible I would like to have the current name kept intact, since Transfusion is highly specialised field. But we also would be interested in other lab sections.

[Effective RDP Leukoreduction]

Why dont you try using the ImmmugardIII filters from Terumo Corporation, if available. They are said to be more biocompatible and the recovery is also better. We have been dealing with these filters for last 5 years and not a single failure on filteration has been reported.

[Sop for cryopreservation using thermo cryomed freezer]

Dear friends,

We are planning to start cryopreservation using the Thermo cryomed controlled rate Freezer. Can anyone help me with the SOP for stemcells cryopreservation.

Thanks in advance

MANOJ

[Centrifuge for Blood processing]

I am also looking for a centrifuge for component separation which has less noise and though good one. Can somebody recommend one? Thanks

Dear F Rahman,

Can u let me know where you are located?

[Centrifuge for Blood processing]

If your requirement is for component seperation the best I have seen is Hettich Rotosilenta 630RS (12bags). Next comes Thermo Herause - 6000i ( for 12 bags), 5500i (for 8 bags).

[Sterile tube connect device]

Dear Watson,

Terumo TSCD is the best in the field. I think you do not have to look for anything else. in fact there are many brands like Genesis, Hemonetics, but nothing compares to the performance and stability of TSCD.

[PRP not clear during the first spin]

hi there,

have you every tried to adjust the RCF of your ref centrifuge? or you just stick to speed of 850g?...our center the first spin for PRP is at spin of 1600 for 10 minutes of spin. there are instances we also experience the same presence of red cells in the plasma what we do is try to allow the red cells to settle for 30 minutes before we spin.

give some feedback if this will work

tonz

Dear Redstaff,

Let me try and will inform you.

Bye Thanks.

[PRP not clear during the first spin]

Seniors, somebody pls help me out

[Sterile Tubing Welders]

the terumo SCD is a rock solid one and worth the price.

[Help on Basic Blood banking]

Pls let me know if u require only the basic requirements for starting a blood bank. Since I am from India I think we may have the same situation.

[NAT error]

Dear emadib,

As per my knowledge NAT is available from Hemogenomics and Roche Diagnostics.

[PRP not clear during the first spin]

Dear All,

I am from Kerala, India. My problem is as follows.

Some of the bags in my centrifuge does not give me a RBC free PRP during the first spin.

Other details. - Centrifugation - RCF=850g, 12 minutes, temp:22 DegreeC.

Bag - Terumo Penpol Triple Bag 450ml with SAGM.

we have been getting clear PRP for last six months, suddenly it has started giving trouble. But surprisingly only few of the bags give the problem, Not all the bags in the centrifuge ( Max.capacity 6 Bags). We ahve checked the rpm of the centrifuge and it is showing correct values as per the tachometer.

Any suggestions from members - could it be a equipment problem?