So does everyone automatically perform an eluate on a positive DAT...on all patients...on only those recently transfused...and how recent is recently transfused? Previously we had the previous 3 months as recent transfusion, but since the majority of antibodies are present in plasma by 20 days post transfusion and most of our eluates were negative or panagglutinins, we opted to proceed to a 30 day rule. Please share current procedures.