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Everything posted by la66
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Good Job Lara!!!:tongue:
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Yes! Just yesterday I had a completed gel panel come to reference lab with only one single dose cell not reacting in a prefect 2+ Jka pattern. I wanted to throw it on the Galileo for rule outs. The panel came back TOTALLY negative! What is up???
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I took a two year specialist in blood banking course at the ARC in Cols, OH, review at Gulf coast and read Technical manual inside and out. After all that I took the test a week after I got back from Tx and I thought I was completely prepared. There was a lot of clinical coag on the test and lots of things right from the Physicians Handbook (which I did not spend enough time on). I took the test in 2001 and had to wait an excruciating week to learn that I had passed! They say, if you think you did fine you flunked and if you think you flunked you PASSED! The review in Tx was the biggest help ever! Lisa B.
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run 2 phenotypically matched cells, if it is still positive then titer them. If titer is >=32 then we call HTLA. I have worked in a reference lab that always tried to id the antibody. That is when it becomes necessary to run ficin/DTT and helpful to know the race. We also tested the antibody ito see if it was neutralizable with pooled serum for Ch/Rg. We then typed the patient for Vel, Lub and Kpb all high incidence abys that can titer just to rule them out.
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our reference lab does a thermal amplitude test and reports to surgeon when there is a non specific cold antibody and when there is a specificity we give antigen negative blood (ie anti-M)