[DNA testing for ABO Subgroups]

jbeauvais,what is the significance of knowing her exactly subgroup? I am not sure, but in my memory I have read some paper about ABO subgroup gene, the same subgroup will have different gene background, some are same as A1 or B, some have change.

It doesn't make any difference in the treatment of the patient, except that it was originally typed as a Group O and sent to us because the forward and reverse didn't match. I also thought that since the B subgroups are "rare" (at least here) someone might be interested in the specimen.

[DNA testing for ABO Subgroups]

Thanks Malcolm

[DNA testing for ABO Subgroups]

Does anyone know of someone researching ABO DNA? We have a pregnant woman who appears to be a B3 Rh positive. In Gel she forward like an O and reverses as a B. There is a slight haze in the Anti B. By tube using Ortho reagents she types 1-2+ mixed field with Anti B and Anti A,B and reverses as a B. From her name she is probably of Far Eastern (Indian) descent.

The only time I have seen a B3 was on my SBB practical, back in the days when they were sending wet samples. I won't say how many years ago that was.

[Problems with cell#2 ortho screen cells?]

We had a problem with Ortho Cell#2 in lot VS286 the first day it was put into use. A 4+ reaction with it. The tech pulled some cells with low incidence antigens (from another manufacturer) and identified it as Mg. The patient's serum reacted with 2 other examples of Mg+ cells.

Jeanne

[IgG cover in transfusion]

That was the perfect paper I needed. We were also wondering if anyone in the US uses an IgG cover for patients with multiple antibodies.

[IgG cover in transfusion]

We recently had a sickle cell patient with a previous Anti-U hyper hemolyze after transfusion of U negative units. In researching this problem we came across a paper from the UK about using an IVIgG cover to transfue patients. Can anyone explain what this is? Is it using the same theory as giving IVIgG to ITP patients?

Thanks

Jeanne in Michigan

[ABO and Rh testing on bag specimens]

Does anyone have a method of cleaning the stem cell bag specimen to get free red cells for typing? Most of the time we can get an ABO type but we have had a least two occasions where we can't get the bag specimen to type correctly with RH antisera. We can't get enough cells to type correctly with Anti-D. The original pherpheral blood sample types as D Pos.

[Making ABO discrepancies for teaching]

I guess I should have tried to be a little more creative with the logon. Can't hide anywhere.

[Making ABO discrepancies for teaching]

I'm having trouble making examples of Anti-A1 for teaching purposes. I've been making them for years using A2 patients with A1 lectin & pt. serum. Lately it seems that when I make and test the sample it's 4+ right after I make them. The next morning the reactions are negative. Anyone have a recipe for making and keeping an Anti-a1 reactive?

Jeanne