Yanxia

Thank you, David. It is a good method, but I can't do it in our hospital. Such a pity!

I don't know if I can use flow cytometry to do it. The question is I can get the survival time of red cells but I can't know whether the cells is destroyed or not, because maybe some cells with less antigen can survive normally, does it possible? Or I can testing survival time in parellel in a patient and a healthy person. I don't know how to do the testing. Would someone give me any suggestion? Thank you!

Thank you, bbbirder. As you say some anemnistic responses( sorry, I don't know what is the meaning of anemnistic. I guess it is the same as second responses, is it right?) can be faster than 3 days, why don't we do the testing less than 3 days if the patient need transfusion everyday or the distance is less than 3 days? Thank you again for the quick response.

I want to test whether an antibody is clinical significant , but I cann't use Cr to label the red cells. Would some friends kindly give me some advice about what I can use to know whether the cells is destroyed by the antibody? Can I use LDH , bilirubin and free hemoglobin? Thank you for your helping!

There is a question about how often we need to do antibody screening test, so I want to know what antibody is induced the fastest than other in the first and second immunization respectively and how long will it need . Thanks in advance!

Johna, the human plasma or serum which we used is self-made( sorry, I don't know if I can call it like this). When we found someone's antibody(healthy donor) titer is very high( often higher than 1024), we will conserve it as reagent. I don't know whether this kind of doing is legal in U.S..

Mabel Adams, I don't know whether you can get the prior donor's sample( the patient had transfused 10 days before). If the antibody is new developed it mybe induced by the antigens of this donor, so it can react with it . If it is possible, you can test the antigen to deduce a conclusion from it.

Thanks Johna for your pointing out ! Please give me a chance to explain it. We perform these adsorption/elutions using human antibodies not the monoclonal antibodies. As we know part of human anti-A and anti-B prefer 4C. I said those things above to prove monoclonal antibodies prefer 4C. Can I get the conclusion that part of polyclonal antibodies prefer 4C? So I think we should test it at this temp, too. Sorry for my English!

We will give A2 or O type cell. (Though I don't think anti-A1 which is not reactive at 37C and coombs testing is clinical significance) I don't think we should worry about the H antigen ,too. Bcause a lot of anti-H or in Chinese people anti-HI is cold reactive and there have not warm reactive induced in A1 people, maybe have but I haved not read it.

In China we will test the eluation at 4C , room temp and 37C Coombs. Because some anti-A and B is prefer cold temp, I think we should do it. I have found some weak A and B antigens react stronger at 4C with monoclonal antibody than at room temp.

Thanks! I always trouble myself with the ABO subgroup antibody's significance. I have not saw any HDFN induced by it and any hemolysis by it. Just as you two said it is better to be careful, and I agree with you in the transfusion selection. But the existent will not always be the right , I need an evidence. Sorry for my stubborn.

A little off the subject， I just wonder if a subgroup patient be gaven the normal type blood , will it have hemolysis？ As we know almost all the antibody of ABO subgroup is cold reactive。 We give pediatric patient the same type blood as themselves though the reverse test is weak (serum antibody ). I have sought for some paper about the subgroup antibody's clinical significance, but I failed.

Today I receive the answer from Biotest AG,it is: Strongly lipemic, icteric or microbiologically contaminated samples may lead to false results. That means that only strong lipemic or icteric samples can cause problems, since strong lipemic material is sticky and the usage of strong icteric material might lead to false results since the quality of the red blood cells is deteriorated. However slightly lipemic or icteric sample materials can be used.

Thanks for your replies. The introduction says it is better to wash the cell before test, so I think the interference will not avoid by washing. The reagent is used in tube and slide method. On-line translators is a good idea， I will try it。

I notice our anti-D reagent says: if the sample is jaundice or fattiness will interfere the result. Would some friends kindly tell me what is the reason and what interference will happen? Because the factory is German, I don't know German. Oh , it is Biotest AG.

I have received help from a friend. Thanks!

Would some friend kindly send Geoff Daniels's Human Blood Group( second edition) page 219 to me? I have missed this page. Thank you! shilysunny@yahoo.com.cn

I don‘t know in USA if the father and the mother all D negative， whether the mother will receive prophylaxis or not. I said that just because I remember some book said it need not. In China we will routinely test D antigen not E e C c, so some Rhnull will be treated like D negative. If one of the parents is D negative with normal RHAG, the other is Rhnull of the regulator type, then the baby mybe a D positive. On 8 month do not give the prophylaxis,then immune the mother.

My teacher said PEG as an enhancement reagent will nonspecific uptake antibodies, so we normally test with a negative control(use AB serum have not irregular antibodies). Mybe it will uptake alloantibodies with autologous cell. I have not use this reagent before, this is just my guess. Mybe there is some method to avoid the nonspecific enhancement which I don't know.

It just remain a mystery to me. I had sent a email to IMMUCOR, but I have not receive the answer. I think it mybe the writer's mistake .

If the autoantibody react more strongly with one antigen like E, what will you do ?

I don't think anti-Ael is the same as anti-AE1. I had sent an E-Mail to the writer, but she is too busy to anwser my question till now. She had mentioned anti-Ael in a forum before the article printed. I will try other way to get the answer, mybe dial to them as Johna mentioned above.

I read it in a paper in Chinese. It said anti-Ael produced by Immucor (I wish I have spelt it right) .

Would you tell me in 'Total Credit Hours: 17 SH 'what the SH stand for ? And the differ between Traditional SBB Certificate Course and Professional Development Course ? Thanks!

Thank you!