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Posts posted by Yanxia
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If a donor have weaken anti-A or anti-B and he/she is not a ABO subgroup, can we explain it as low-globulin.
I wonder if a low globulin person can donate or not.
Need some help and thanks for attention.
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Thank you , Mabel.
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I remember the lifespan of platelet is nine more days. I am very interested in what is about the function of seven-day shelf life apheresis platelet. Thanks for some helping.
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This few days I read some papers about Nitric Oxide ,I have a question about whether the acting Nitric Oxide is in red cells or in plasma. I think it maybe in red cells. It is such a pity that I can't get the original article about Nitric Oxide's function in blood transfusion, I don't know they test what part of NO. This kind of gas in body can produced by endothelium and other cells, and lots of factor can stimulate body to produce it, especially in trauma and infection so why the transfused cells can't absorb it in circulation if they can easily diffuse to cells in pre-transfusion charge?
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Thank you, Jschang. I am very happy to meet you here.
I have checked the reported Del phenotype, I can't find ccEe with Del positive which I remembered have read in some book. Maybe I am wrong.
One day I phoned Mr. Xiang, he is my teacher in Shanghai. He said adsorb and elution test is a very difficult test which frequently get fale positive result.
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I will call this patient Rh negative, if he/she is a donor, it is Rh positive. If patient OB will not give Rh0Gam according to some rule, but I think in this kind of case it is better to give prophylaxis. I don't think immunize by baby's cell is differ from transfused cells. Don't give prophylaxis in weak D maybe because the economization.
This just my view, opposed view is appreciated.
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Thank you, Linda!
Thank you, Mabel!
Del is a very hot topic in China recently, it is under investigation, we do weak D test use 3 kind of anti-D in IAT, not do Del test routinely.
I encountered a pregnant women, her blood type is ccEe and D negative. There are anti-D and anti-C in her serum, because the sample is exhausted, I can't detect if anti-G in it.
I do the absorb and elute test, the result is not good, I run two kind of anti-D reagent the same time, one is + -,the other is negative.
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We will report it as Rh-negative and will not do weak D test for it .
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Thank you, David Saikin.
Original Du is divided into Dweak which express all D epitope and D partial which will lack one or several D epitope. This is in China's text book says, maybe we have the different name of it.
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This is a interesting finding, I think it will open a new era of blood transfusion.
Before adding NO , we need know what is the proper quantity of NO in red cells, avoid dilate the vessel too much, result in haemorrhage or other bad case.
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Dweak person can't produce anti-D, I don't know if Del have all the D epitope as Dweak.
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Rcurrie, I learn lots of professional knowledge from your posts. I always think you are a good technologist and wish myself can do this work as good as you. We will miss you.
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Yes, my meaning is bacterial infection in his blood stream.Shily,
If your reference to "blood poisoning" is meant to mean that he has a bacterial infection I would suspect that this could be the source of the problem. Bacterial enzymes can certainly have an effect on red cell antigens.
Days later I meet another neonatal blood is also mixed filed, he is an ABO HDN infant.
I suggest them retest blood groups after one year old.
Thanks for the replies, I learn some knowledge new to me.
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His father is B positive, his mother is A positive. Both of them are antigen normal. His mother is A1 type, and the patient is A1 1+mf.(mix field, s is strong, w is weak)
If my depiction make inconvenience to you, I am sorry for that.
His father and mother is physiological parents.
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I have just received a sample from a 2-month boy, he is a blood poisoning patient.
anti- A 3+s mf ; anti-B 3+w mf; anti-D 3+; anti-C neg; anti-E 3+.
Ac neg ; Bc neg;Oc neg.
He has not been transfused before and he is not twin.
I just heard he is premature delivery 2 months before mature.
I can't explain his blood type, it seems not a two population blood cells case, and no report of this kind of disease can cause antigen weaken, maybe because he is so young,
I don't know.
What do you think ?
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I had luckily met a donor, his is a para-bombay and Se-weak person. His Lea and Leb antigen is less weaker than positive control.
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For an auto immune patient maybe to test his Lea and Leb substances in serum is a way to explain no hemolysis of his red cells if there is no sign of hemolysis.
Oh, I remember a question, in Caucasian the frequency of type Le(a+b+) is zero, maybe I am wrong.
We all know sepsis can uncover the T antigens, I don't know if the reagent have something to react with this kind of polyagglutination antigens.
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It is so odd!
I have a daring guess, it is when the red cells' volume is smaller than normal and the antigen is weaker, when reacted in Gel, it is slower to form a larger conglomeration to be arrested by the Gel sifter, so we get the result that Leb is weaker in Gel than in tube, and for the Lea it seems normally.
Another guess is the difference between the reagent in Gel and in tube.
OPUS104, you said All reactivity is removed when treated with Lewis substance
, I know less about this, can you tell me what kind of substance you use, it is Lea or Leb or both can removed the reactivity. Thanks!
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I don't know if I said clearly of my meaning. I want to know the result of autocontrol or DAT just because I think if the patient is lea postitive and produced antibody against this antigen one reason is autoimmune , the others are partial antigen and cross-reactive antibody but not with his own antigen.
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I get so many reply, what a pleasant surprise! Thanks a lot!
I said things above just want to know if the patient weaken or lost his antigen, and at the same time he produce antibodies against his cells. Maybe it is DAT in U.S. and patient's Lewis type now.
Yes, Lewis antigen can lost in the situation rcurrie mentioned above. I have several to add. They are various forms of cancer( pancreatic, gastric, colorectal, bile duct, bladder), and severe renal disease.
Francois et al. had tested 14 anti-Leb sera with synthetic oligosaccharides, found that four cross-reacted with Lea trisaccharide.
So I guess this antibody is produced when disease weaken his Lea antigen or it is a cross-reacted antibody with Lea antigen.
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rcurrie,would you kindly explain the relationship between anti-le3 and Duffy system? Thank you!Sounds like an anti-Le3 (that's a Duffy system joke!).
BC
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Excuse me, can you tell us about the auto-control? And what kind of disease he is?
Maybe it is a new developed antibody with cross reactivity.
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Thank you, ,Mabel.
I have not saw any A&B anti-subgroup antibodies which is IgG. If a mother is A2 or Ax have naturally produced anti-A1 , during her pregnancy the baby's cellscan enter her blood circulation stimulate the B cell to produce antibodies. As we have not saw any this kind of IgG antibodies in subgroup people. Would I get the conclusion that A&B subgroup immunology can't chang the antibodies to IgG, they have not the ability of evoke B memory cells?
In China I have not saw any reports of warm reactive subgroup antibodies, not like U.S., this maybe the difference between human race, So I am very interesting in this antibodies' investigation.
can we explain a donor as low-globulin
in Immunohematology Reference Laboratories
Posted
Thanks, Anna.
Because English is not my mother language, I express my meaning not so exactly, I am sorry for that.
My meaning is if a person have reduced globulin, he/ she my have symptom that let him/her to feel not a healthy person. I think a person to donate blood will be self-feeling fine.
Yanxia