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Yanxia

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Everything posted by Yanxia

  1. Yanxia
    Mabel, if my memory not cheat me there has acquired A antigen, it is a kind of T polyagglutination, been agglutinated more strongly in B serum.
  2. Yanxia
    Can saline replacement reduce the strength of significant reactivity, I think this is a question.In AABB Technical Manual suggest to use saline replacement to distinguish the real agglutination from rouleaux, I think spin the tube then spill it gently on a piece of glass( sorry for my poor English), then see it microscopically. The rouleaux is stack of cells ,its edge is smooth seems like been surrounded by fibrin or grume.
  3. Yanxia
    Thank you, Mabel.
  4. Yanxia
    We will use stored less than 7 days cells for cardiac patient, because the better flexible and lower Potassium. I have not read the article in the NEW England Journal.
  5. Yanxia
    ROsemary, would you tell me the meaning of "Cold Aggs", sorry I can't find the explaination,thanks!
  6. Yanxia
    I think rouleaux and cold auto( titer below 1000 in 4 drgree C) are all insignificant, the question is correct name it, because they are two different reaction.
  7. Yanxia
    I guess the baby have ABO HDFN and other type of HDFN. In the newborn's blood stream, the cells with stronger A&B antigens had been destroyed,so it have a temporary balance.The transfused cells destroyed more than the newborn's cells because the adult's cells have stronger A&B antigens than newborn's. Maybe the strong DAT is not due to ABO antibodies.
  8. Yanxia
    Bob, can you remember how strong of the ABO HDFN baby's DAT?
  9. Yanxia
    The enhanced DAT test will examine the result microscopically. In Chinese people there is not ABO HDN its DAT stronger than 2+ macroscopically, so see your post I feel surprising.
  10. Yanxia
    Thanks Mabel! Galvania, you have seen 2 cases of ABO HDN ,the DAT of the baby was strongly positive.That is so strange to me to hear that. Did you do the enhanced DAT test?
  11. Yanxia
    It is my pleasure.
  12. Yanxia
    In our hospital , there will be 3600 babies be born every year, and in those babies just one or two need exchange transfusion. In the 3600 babies the O mother with not O baby is more than 30%. When I study in blood center, they will encounter ABO HDN very often,the teatcher said there will not strong DAT in ABO HDN, because the baby's antigen is weak and the soluble blood type antigen in lots of person's plasma.
  13. Yanxia
    Almost all the ABO HDN I know its DAT is not stronger than 1+. If this is differ to you have found, maybe because the anti-globulin we used is different or the method. We just wash the cells three times then suspended in saline to 3%-5% concentrate, and one point cells to two points reagent, immediatly centrifuge, then monitor macroscopically. Tomorrow is China's lunar new year, happy new year!
  14. Yanxia
    If the red cells have stronger B antigens be destroyed in the cord cells then we get the neg DAT result, the 2nd sample is one day later there were new cells be produced so DAT is weak pos. This is just a hypothesis. In China a majority of ABO HDN have weak or neg DAT, we du heat elution, DAT and plasma antibodies test to detect it, the most important is elution.
  15. Yanxia
    Thank you, Mabel. You are my good teacher.
  16. Yanxia
    In vitro test we will use the antibody's preference of bind complement to find it. I don't know if in vivo it will have the same ability. If it is like in vitro we use anti-IgG may miss it. Yanxia
  17. Yanxia
    I agree with Galvania. Kidd blood group antibodies can produce HDN, I don't know if the baby's DAT positive for C3 or IgG or both.
  18. Yanxia
    Oh, there is a question I forget. In China the D neg peole's frequency is 4 in 1000 peole, and in those D neg peole 1 in 3 is Del which may not produce anti-D, and because the price we don't use RhIG routinely. I suspect the reaction in the mother is first immune, because her IgM and IgG titer is so low.
  19. Yanxia
    Thanks. I learnt lots of things in this post, I feel very happy to read all the replies. The baby had not done exchange transfusion, he is fine, and he is delivered through vaginal. We have not test anti-A in the baby's elution. In China we use the screening cells made in Shanghai Blood Center China which include Mia antigen. In China Han ethnic people part of ABO HDN need do exchange transfusion and some just need blue-light cure, lot of just have jaundice which is a little severe than normal need no medical cure.
  20. Yanxia
    Thanks to all the reply. I asked this question just because one of my friends said that irradiation can't make all the lymphocytes lost its ability of dividing, he think leuko-reduce is safer.
  21. Yanxia
    I have a question: some antibodies will have a better reaction when complement is present( i.e. Lewis and Kidd), so sometimes we use anti-complement to detect this kind of antibodies. Can this exist in newborn's blood stream?
  22. Yanxia
    There is a mother is B neg, have a 4 years old son. The newborn is AB pos. The anti-D in mother's blood titer is IgM 1 and IgG 2. The cord blood is weak positive and elution anti-D is weak, too . I wonder this is first-immune or memory reaction. If the prenatal antibody titer is weak can it indicate the HDN is mild? Is ABO HDN plus Rh HDN serious or weaker than Rh HDN lonely? Can we use the same type of blood as the mother to do the exchange transfusion( compatible with the mother serum antibodies)? If we can do like this, what is the meaning of eluate the baby's blood cells? If prenatal antibodies titer is too high, Caesarean birth or not Caesarean birth( sorry, I don't know how to say the birth without surgical interfereance) will be better to the baby? So many questions just because a case, I look forward to any help.
  23. Yanxia
    We in China will draw the sample through another arm's vein. I think the lab value will affected is calcium and kalium.
  24. Yanxia
    I don't know if there is some new point of view that prove it can. Thanks for some advice.
  25. Yanxia
    Thank you,John. Sorry for my poor English, so if my understanding is not right, please forgive me and point it out. If the person had been transfused before , the allo-antibodies may disappear during the time, the cross-match will be compatible. After transfused the former 2 unite of RBC may evoke the memory reaction, so I think it is better to re-crossmatch the remaining 2 unite of RBC.
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