We routinely screen using both LISS and gel methods and perform a DAT by tube method. Recently we had been asked by a customer to perform the autocontrol in Gel so as to duplicate their results. Our tube DAT was negative----and usually will be unless the autocontrol in Gel is strongly positive (3+ or more). Our tech performed a DAT in Gel (1+) and by tube (negative). An eluate, tested by tube method, was negative. Out of curiousity we then performed an eluate in Gel and it was also negative. Routinely we do not perform eluates in gel. The patient did have an alloantibody (Lea) and units were crossmatched using the gel IgG card. I tend to agree with the thinking that the gel system is too sensitive to give clinically meaningful DAT reactions at 1-2+.