I read this as washing with room temp saline is sufficient to remove reactivity from cold-reactive antibodies. Some strong ones may require washing with warmed saline, but you risk losing some clinically significant antibodies. In practice, most of our cold antibodies picked up in gel do not require pre-warm and it's unnecessary extra time. Our current procedure is to go straight from our most sensitive method (gel) to our least sensitive method (PW PBS-IAT) and I'd like to change that! Thank you @snance for the references!
