We prepare our elutions with Immucor's Elu-kit and have validated them in gel, which works great - both in testing and volume needed for the antibody identification. However, recently, we have seen a few interesting cases in which the patient is in the process of making an antibody and the antigen positive cells react with definite positive reactions (2+). However, on a majority of the antigen negative cells, there are definite junky, repeatedly-reactive, weakly positive reactions. After several weeks, the eluate 'settles down' to just the original antibody identified and the junky reactions are gone. Has anyone else had this experience or can explain what we are seeing?