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SbbPerson

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Everything posted by SbbPerson

  1. I just answered this question. My Score PASS  
  2. I just answered this question. My Score FAIL  
  3. Are you using the Biohit pipette in a manner that may compromise the accuracy of the volume delivered? Does the pipette instructions indicate that it is okay to use at a slight angle? As Elinf has mentioned, how do you explain automated gel card readers? Do they use the air gap method? Thank you.
  4. On a previous new post, someone said the Ortho Representative told him they needed to do the air gap method. If Ortho is requiring the method, it would be nice to have some printed material about it. If there is none, it’s no big deal, I think I’ll live.
  5. I have never seen the pipette used any other way than vertical. I was just looking for printed materials for this air gap method, if any exist, so I can have something official for my reference. Thank you 🙏
  6. Since a representative from Ortho says we need the “air gap”, does Ortho have actual printed material with instructions on how to do this air gap method?
  7. I know this thread is really old. I was wondering if anyone else does this technique and what is your principle behind it.
  8. Welcome! You came to the right place! The members here are experts in BB and quite knowledgeable in the field. Good luck with your studies.
  9. It has been over 100 years since the ABO blood types have been discovered, but we still don't really know what are their "function"? What exactly do they do? What did they evolve from? Thank you
  10. I doubt that would be likely, since I think a positive antibody screen disqualifies donors. But maybe I am wrong.
  11. In plasma products, what exactly would "spike" the formation of Anti-D? Residual D-positive RBCs or platelets?
  12. You wouldn't happen to know the Fisher formula or where I can find it? I am just curious about the actual math/statistical tool(s) involved in the rule of 3. Thank you.
  13. Rarely you will find an WAA stronger than an underlying alloantibody. That is what makes this method useful. The weaker autoantibodies won’t react and only the stronger alloantibodies will react. Attached is the AABB sop for this method. METHOD 3-2. SALINE INDIRECT ANTIGLOBULIN TEST PROCEDURE.pdf
  14. I know this thread is several years old but I just want to say I agree with Malcom. Also, i just want to add that I have been researching this method for the last few days, and found out that many labs use this method. The main idea here is that alloantibodies usually have a stronger reaction that WAAs. So if you don’t use saline without any enhancements, you will only get the alloantibodies reacting. This especially works well when the underlying alloantibodies are 2+ or greater and the WAAs are 1+ when done with enhancement. It’s a “quicker” way to identify alloantibodies without doing an autoadsorption.
  15. Thanks everyone for your input! I found the method on the AABB website. It's attached if anyone wants to see it. Thanks again! METHOD 3-2. SALINE INDIRECT ANTIGLOBULIN TEST PROCEDURE.pdf
  16. This the tube method? Thanks so much ! How many tubes do you use for your panel ?
  17. Thank you for your response. What is the ingredient in this saline? Do you know the manufacturer name or the procedure steps? Thank you.
  18. Thank you for your post. I am sorry, but what is NSF? Do you know the name of the manufacturer of this saline panel? or know of any sources about saline IgG? Thank you for your time and help.
  19. Anyone here has any experience with saline IgG and it's use in detecting underlying alloantibodies? In a situation that may involve possible multiple alloantibodies or warm autoantibody(ies). I would like to find out more about the principle and the procedure behind this. Thank you.
  20. Thank you Malcolm. Would you have any sources/web sites on this subject? I would like to read more on the subject if possible. I tried googling it but with no success. Thank you so much!
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