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Posts posted by SbbPerson
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I apologize if this is a dumb question, but I can't seem to remember the answer. You know when sometimes a person is scheduled for surgery and so their type and screen expiration date is extended to the surgery date plus 2 or 3 days. One of the requirements in order to extend the expiration date is that the patient has no transfusions or pregnancies in the last 3 months. What is so special about 3 months? I know this is probably a dumb question. Thank you in advance.
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I used the WASP several years ago. At the time, there were only 2 hospitals in the country that used it. I happened to work for one of those hospitals. The WASP did everything, plated the cultures, incubated them, discarded them, took pictures of the cultures, and also made the gram stain. They were beautiful slides, perfect grams stains, just like you would expect from a machine.
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please disregard my post.
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Erroneous post. Many apologies, thank you.
- BldBnker and David Saikin
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May I ask what type of audits are they? Are they blood bank patient results audits? Or are they general laboratory procedure audits?
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There are 3 key pieces of the validation process you must inspect and "validate":
1) Your hardware : printers, code readers, etc..
2) software: (your bloodbank and/or laboratory information systems)
3) User performance: mechanism to test the user's ability to understand and correctly operate your electronic crossmatch process.
I attached an old copy of the FDA guidance for computer crossmatch. Good luck.
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On 12/8/2020 at 10:55 AM, Lauro said:
I must be missing something. Slide 48 lists that the zeta potential is inversely proportional to the ionic strength of the medium and density of the ionic cloud. That would mean that a low-ionic strength solution (LISS) increases zeta potential. Since the zeta potential is basically an indicator of electrostatic repulsion between similarly charged particles, that would mean that LISS would increase the electrostatic repulsion between RBCs, which, I believe, is not the case.
I guess I'll have to go back and brush up on my chemistry.
I thought it was straight forward. RBC surfaces is negatively charged. LISS is positive (though a "low" positive). You increase the positive charge (the sodium ions) it dissipates the negative charges of the RBC surfaces, thus lowering the zeta potential between the cells. And more sodium ions, the bigger the ionic cloud.
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I apologize, I have never used Softbank, but I am familiar with reconstituted RBCs. I assume when you say Reconstituted RBCS, you are talking about adding plasma to red cells to get a desired hematocrit. The attachments is from the ICCBBA website. It tells you how to get an FDA compliant product label for reconstituted red blood cells.
Also, there are simple math formulas to figure out how much plasma you need to add to RBCs in order to get a desired hematocrit. But your software should be able to handle the math, all you need are the numbers for your product label.
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Thank you for update 👍
On 5/11/2022 at 11:20 PM, yan xia said:Here is a follow up. The baby had tested with anti-A on 27, April and transfused with O washed cells the same day, but on 10, May she had no anti-A and received A type packed red cells with no transfusion reaction. She has stoped feeding on her mother's milk for 14 days.
I tried to persuade her mother to do some tests. The results came out and she has no anemia, with normal reticulocyte count and percentage. Her bilirubin and LDH are normal too. But she has not tested her haptoglobin.
She has not received any blood transfusion and IVIG. She just diagnosed with slight anemia during pregnancy and after birth. She had taken iron supplements during pregnancy,.
This is her eventh pregnancies and the third baby, she had several miscarriages. She told me her case seems like a mystery both to her and the doctors she know. She just want to know if she is ok and the baby will be healthy in the future.
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This link lists some "high level" changes to the 2021 AABB Quality Systems Framework.
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Yeah, joint commission actually checks for that. It's because cardboard boxes or shipping containers can carry dirt and other contaminants and introduce them to new environments. Is there a room where you can store the saline boxes where the plebs don't go in and out of? Maybe put a sign outside your blood bank storeroom, "No phlebotomist allowed". We have used saline in cardboard boxes for many years, but phlebotomists usually have no business to go in our blood bank storeroom.
The safety committee's job is to be familiar with joint commission and OSHA standards and regulations, and patient and employee safety is their first priority. There is probably a "technicality" somewhere you can find that might help you, good luck.
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Welcome Tim O'Neil
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Welcome Oli Molina.
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Welcome Alex Av.
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You can use a simple FFP dosage formula or online calculator. There are many of them on google. Of course, this is not a solve-all , and other clinical indications must be taken into consideration when administering FFP or any blood product. Good luck.
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The mom is probably a subgroup of Group A, perhaps she is A2 type. If so, she probably has Anti-A1. Subgroup A cells will react with Anti-A reagents.
From first read of your post, I was thinking maybe the Anti-A1 was passed through the breast milk. But if I am not mistaken, Anti-A1 is an IgM antibody, and those don't usually get passed through breastmilk. Let me dig further into this and see what I can find. Good luck.
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It has always been an unspoken rule for me to gently mix it once before every centrifugation. For the Jka/b, you mixed it once then incubate and then centrifuge.
For the CEce you mixed it once for the IS before centrifuge. Then for the RT incubation part, you mixed it once before centrifuge.
The gentle mixing before centrifugation allows cells to resuspend and bind to any possible "unbound" antibodies and then latticed together in the centrifuge.
I attached the Ortho Instructions for Use for Anti-CEce. Good luck.
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I believe the product code doesn't need to change if it's directed donation. But of course if it was autologous, it needs to be changed.
https://www.iccbba.org/isbt-128-basics/frequently-asked-questions/national
- David Saikin and Mabel Adams
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I have never used the Vision, but I googled it and looks like the FDA did a carryover study on it. I attached it. It is from 2019, sorry. Maybe you can find something more current. Good luck.
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We only use Hematrax just in the event that our LIS is down and unavailable. You have no LIS for your lab? So your lab results are handwritten? I can give you instructions on how to use it but I would need time to dig them up. I will if you still need help. Good luck.
- jayinsat and JJSPLAYHOUSE
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Have people ever had to rule in or rule out cold antibodies based on reactions you get from a gel card panel/screen?
For example in a case where you get a discrepant ABO result because a cold antibody is messing up the reverse typing.
Anyone with any knowledge on this? Thank you.
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16 hours ago, Malcolm Needs said:
Welcome COWEN4.
Hi Malcom, I attended your presentation on ABO discrepancy. Thank you, it was a very informational presentation! Thumbs up!
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On 4/4/2022 at 5:25 PM, Mabel Adams said:
We have for IgG XM but not IS. The latter is very fast in tube and we don't do it except during computer downtimes and some rare occasions. We use the electronic XM for most.
Same here. It wasn't that long ago when Electronic XM's weren't a thing. I find it so amazing how far technology has "improved" blood banking in a relatively short time.
Infrared Thermometer
in Transfusion Services
Posted
I haven't seen an infrared one where you can lay the unit down on it. Most I have seen are handhelds ones, where you shoot a laser at the blood product. Anyways, I found one that thermocouple thermometer where you can lay the unit on it. It is called the Temp Check, and the manual states it is pretty accurate. It is attached. Good luck
TEMPCHECK-MANUAL-V1.pdf