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Posts posted by Bijoux71
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Good afternoon, I hoped to weaken or destroy unexpected (w+) reactivity observed with [2 of 6] cells at LISS-IAT in allo-adsorbed plasma. The CDP (Chloroquin Diphosphate) treated (120 min) cells appeared to have enhanced the plasma reactivity to (2+) at LISS-IAT. Obviously, there is known info about antigens sensitive to CDP treatment but I have not found any mention of blood group antigens enhanced with this chemical. I may try to repeat the CDP treatment with a shorter incubation time, or a 1:3 (cell: CDP) ratio in case it is an overtreatment issue.......Any thoughts or suggestions?
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In scenarios like this, questions and possibilities that first come to mind are the following, not in any specific order:
1.) The DAT of the donor unit is extremely relevant. Yes, DATs are not routinely run on all donors however, when IRL testing discovers a discrepant IAT antigen type, Rh or other, a positive DAT is often the culprit. If the discrepant type is at immed spin or direct agglutination, several possibilities come to mind, such as a bacterial contaminated donor unit which may cause spontaneous agglutination, or a medicine that donor takes and is not screened for or donor doesn't give an accurate reply to pre-donation questions, or a donor with a cold agglutinin.
2.) Some previous posts have stated that most current Anti-D reagents will not detect DVI. I feel like that statement is confusing and should be worded to say "most Anti-D reagents will not detect type DVI by direct agglutination, but are able to detect DVI at IAT.
- carolyn swickard and Yanxia
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Stumped by CDP
in Transfusion Services
Posted
Patient cells were DAT IgG +