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Everything posted by srichar3

  1. Chido and Rodgers can be neutralized with Chido/Rodgers positive plasma, what are the antibody characteristics that suggest such an antibody that make you decide to do the antibody neutralization? My understanding is they will be pan-reactive with most if not all identification panel cells and non reactive in enzyme, are there any other characteristics that should lead to the decision to try neutralization or should it be performed on any pan-reactive, non enzyme reactive AB? Thanks
  2. 5.3 Timing of samples The maternal sample for FMH estimation should be taken when sufficient time has elapsed to allow fetal cells to be distributed within the maternal circulation following delivery, manual removal of placenta or sensitising event. A period of 30-45 minutes is considered adequate (BCSH 2006a). BCSH FMH Guidelines 2009
  3. We have a blood transfusion administration module in our HIS, on receipt on the ward they have to scan the unit against the patients electronic record, if it doesn't match the patient it was issued for, it will not allow them to proceed with the administration. I am working to further enhance this by incorporating patient wrist band scan at the same time at the patients bed side.
  4. I want to introduce the Rosette test for FMH screening, unfortunately no commercial kits are available in the UAE where I am based. Right now I cannot even find a Keilhauer staining kit. Does any one use an in house method for this test? if so what Anti-D reagent do you use? AABB have a method which just states high protein Anti-D reagent, can anyone offer any further advice on selecting a suitable Anti-D antisera for use with this method? Thanks
  5. I am looking for a method for performing Hemolysin Titres on blood components, does anyone have one they would be willing to share? Thanks
  6. The thirty year rule came from the European blood directive 2002/98/EC, the NHS is only UK based, this was a European wide requirement. https://www.health-ni.gov.uk/articles/blood-safety-and-quality-regulations-2005-amended#:~:text=The Regulations,-The retention periods&text=Blood establishments and hospital blood,(regulations 8 and 9). Also there is no such 30 year limit on suing the NHS, its 3 years from the date of discovery, so if you didn't find out until 50 years later then it would be 53 years. Applicable Time Limits To Sue The NHS Claimant immediately aware that negligence had caused avoidable harm 3 years from the date of the negligent act Claimant made aware later that negligence had caused avoidable harm 3 years from the date of the discovery Those without capacity No limit (There are some limits to those claiming on the injured party’s behalf – please see later sections or call our team for specific guidance)
  7. Wasn't 30 years due to vCJD risk? as the incubation period can be 20 years+ hence records need to be kept this long at least for tracing potential donor and other recipients from same donor? With regards to record keeping, in my experience in the UK while we kept the administration record of the anti-D the same as we did for all blood components/products, 30 years. Once we were satisfied that the anti-D was not of immune origin we would remove the record from the antibody file otherwise we would not be able to use electronic issue for future crossmatches. Our LIS was such though that it would stay in the audit log of the patients file indefinitely that it was added then removed.
  8. And also my reason for asking which antigens are most likely, so I know which ones to try first rather than ordering them all.
  9. I'm looking into sourcing these from a company in Germany called inno-train who provide molecular blood typing products also, I believe from the name in the IFU's provided these are the same (imusyn GmbH & Co. KG.) that you mention above. My next question was going to be if anyone has any feedback on these techniques? imusyn rBGA Kits_v19_EN_RUO.pdf
  10. Column agglutination with glass beads instead of Gel from Ortho.
  11. We run auto control with all our panels and in these cases they are negative, were a women's speciality hospital in the middle east. I'm literally getting 3 or 4 of these a week and we have no reference service so mostly they are going unidentified. I'm trying to develop our identification protocols and source additional reagents to help ID these cases but struggling to find HFA negative cells out here. Just to add in most cases these are pregnant women.
  12. We receive a lot of antibody screens that tend to be positive in all screening cells, both with or without enzyme reactivity. In such cases what are the most likely culprits?
  13. Is there any Alinity HQ users who have results evaluation report for CAP survey FH9-B who would be willing to share a copy of the report? we are evaluating Alinity HQ and want to Alinity group means. Thanks
  14. Yes from the UK originally but now working in the UAE where we follow AABB and CAP standards. Are you from the UK also?
  15. In the UK its common practice, every hospital I have worked at follow this practice, it is strictly for life and death emergencies where there simply isn't time to lease with the blood bank. I haven't seen anything in the CAP or AABB standards regarding it that's why I was asking, I guess under US regulations/practices this isn't a followed practice then. Regarding the vending machines these are more a sort of remote electronic crossmatch than emergency O neg I believe.
  16. O Neg that is kept in a fridge that nurses/doctors can take in urgent situation without been crossmatched or issued to a patient.
  17. Just want to see how many labs out there use flying squad blood and if so how do you manage it? does your LIS have a process built in for managing such situations or do you have a manual process where the documentation is resolved later? Thanks
  18. Maybe I'm reading your question wrong but why do you need to use XM profile? we do antigen typing for all antigens on our vision and the Vision has profiles setup for all the sera it offers. Ortho also has the option for other manufacturers sera and these can be setup in the UDP (User defined protocols). You have to titre the sera to ensure it has a tire of less that 1:1024 as this is what Ortho claim is the limit of their analyser for carry over.
  19. Found an old Bio-Rad card that is still in date so decided to give it a try in that and its shows a plane old B Pos! But the Ortho gives a consistent 1 or 2 + A reaction in 3 different cassette types. Awaiting an explanation from Ortho.
  20. The A2 was done in Gel, or rather glass beads as Ortho is out here. The left well is A2 and the right is A1 so clearly weaker reaction with A2 but still reacted. I also repeated the tube method and when I did it, A1 gave strong reaction and the A2 was barely visible by eye by but large agglutinates observed microscopically. Any suggestions for further tests I can do on this one? or would genetic testing be the only way to resolve this now?
  21. I would have to check with the tech who performed it.
  22. In your experience does the fact the Anti-A is reacting with A2 cells rule out A subgroup of A been present?
  23. I meant it in the sense that it's an antibody reacting with the A antigen rather than another cold reacting antibody reacting with another antigen on the Acells been the cause. However as per my original post it did react with A2 cells this along with the strength of the back group was why I was questioning and AsubB.
  24. I tested the back group against 4 of each ABO group, Reacted 3+ with all A and AB samples and Negative with group O and B so its defiantly an Anti-A and not another cold antibody interfering. Also did A1 lectin and that was neg..
  25. AABB mandates the giving set are Pyrogen free, it appears the ones we use are Endotoxin free however they have advised that this is not in compliance with the standard as not all Pyrogens are endotoxins. Just curious what giving set other site that are AABB accredited are using? Thanks
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