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April 2024 Challenge

David Saikin

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Status Replies posted by David Saikin

  1. cbaldwin

    Hello David,

    I think several months ago I read a post where you stated you only had anti-IgG and did not use anti-IgG,-C3d.  Is my memory correct?  I carry both anti-IgG and anti-IgG,-C3d--the anti-IgG,-C3d for the rare transfusion reaction workup.  I would like to stop carrying the anti-IgG,-C3d because the vials come in packs of 10 and out date.  Do you have any advice on this for me?

    Thanks!

    Catherine Baldwin

     

    1. David Saikin

      David Saikin

      If you go with the anti-Comp antisera you'd have to purchase the check cells that go with it.  Immucor's are a lot less expensive than Hemobioscience's (I didn't tell you that) and now Quotient is also distributing them.

    2. (See 2 other replies to this status update)

  2. cbaldwin

    Hello David,

    I think several months ago I read a post where you stated you only had anti-IgG and did not use anti-IgG,-C3d.  Is my memory correct?  I carry both anti-IgG and anti-IgG,-C3d--the anti-IgG,-C3d for the rare transfusion reaction workup.  I would like to stop carrying the anti-IgG,-C3d because the vials come in packs of 10 and out date.  Do you have any advice on this for me?

    Thanks!

    Catherine Baldwin

     

    1. David Saikin

      David Saikin

      I only have tube anti-IgG.  I also carry anti-C3b,-C3d in vial.  I do my DATs in gel, using the IgG gel card and I use the anti-complement antisera in the buffered gel not in tube (the C check cells are always 4+).  I don't buy the polyAHG gel for the same reason - I'd outdate more than I'd use.  You should be able to find a vendor that sells polyAHG in less than 10 packs.  Ortho probably does vials of 3 and you could check with Quotient Biodiagnostics and BioRad also. I don't recall if Hemobioscience is distributing it.  You could also just get the anti-complement antisera - Ortho sells me in single btls, though I usually purchase 2-3 at a time.

      Hope that helps.

      Dave

       

    2. (See 2 other replies to this status update)

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