Lilly Petkovic

On behalf of a CAP accredited lab, the following question was received: "We have been using pink top tubes for about 5 years in the blood bank. We got to thinking that calcium is chelated in these tubes. We were further thinking that calcium is needed for complement activation. So, we wonder if when we do antibody screens, panels and crossmatches whether we will never see hemolysis even when an antibody is present which would have caused hemolysis when we used clot tubes. Does anyone know??